Singlet oxygen production by human eosinophils.
Singlet oxygen production by human eosinophils.
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发表时间：
1988-07
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通讯作者：
J. R. Kanofsky;H. Hoogland;R. Wever;S. Weiss
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作者：
J. R. Kanofsky;H. Hoogland;R. Wever;S. Weiss
人嗜酸性粒细胞经佛波醇肉豆蔻酸酯乙酸酯刺激后，被发现可产生单线态氧特征的 1268 nm 化学发光。单线态氧的产生需要溴离子的存在。 100 µM 的溴离子浓度（与全血的总溴含量相当）足以使嗜酸性粒细胞产生可测量量的单线态氧。对于所使用的条件（10(7) 细胞/ml 和10 微克/ml 佛波醇肉豆蔻酸酯乙酸酯），单线态氧产生的持续时间很短，大约5 分钟，并且单线态氧的总产量适中，为1.0 +/- 0.1 microM。单线态氧停止产生后，细胞仍然存活。这是活细胞产生单线态氧的首次演示。嗜酸性粒细胞产生的单线态氧可能是由过氧化物酶催化机制产生的，因为纯化的嗜酸性粒细胞过氧化物酶-过氧化氢-溴化物系统也被证明可以产生单线态氧。嗜酸性粒细胞过氧化物酶的独特性质可以通过以下事实来说明：在 p2H 7.0 和 100 µM 溴化物下，嗜酸性粒细胞过氧化物酶产生的单线态氧理论产量为 20 +/- 2%，而在相同条件下，髓过氧化物酶和乳过氧化物酶分别仅产生 1.0 +/- 0.1% 和 -0.1 +/- 0.1%。
Human eosinophils, stimulated with phorbol myristate acetate, were found to produce 1268 nm chemiluminescence characteristic of singlet oxygen. Singlet oxygen generation required the presence of bromide ion. A bromide ion concentration of 100 microM, comparable to the total bromine content of whole blood, was sufficient for the eosinophils to generate measurable amounts of singlet oxygen. For the conditions used (10(7) cells/ml and 10 micrograms/ml phorbol myristate acetate), the duration of the singlet oxygen generation was brief, about 5 min, and the total yield of singlet oxygen was modest, 1.0 +/- 0.1 microM. The cells remained viable after the singlet oxygen production ceased. This is the first demonstration of singlet oxygen production from living cells. The singlet oxygen generated by eosinophils likely results from a peroxidase-catalyzed mechanism, since a purified eosinophil peroxidase-hydrogen peroxide-bromide system was also shown to produce singlet oxygen. The unique properties of eosinophil peroxidase are illustrated by the fact that at p2H 7.0 and with 100 microM bromide, eosinophil peroxidase generated 20 +/- 2% of the theoretical yield of singlet oxygen, whereas under identical conditions, myeloperoxidase and lactoperoxidase produced only 1.0 +/- 0.1% and -0.1 +/- 0.1%, respectively.