CLONING AND EXPRESSION ANALYSIS OF TRANSCRIPTION FACTOR RrTTG1 RELATED TO PRICKLE DEVELOPMENT IN ROSE (Rosa rugosa)
CLONING AND EXPRESSION ANALYSIS OF TRANSCRIPTION FACTOR RrTTG1 RELATED TO PRICKLE DEVELOPMENT IN ROSE (Rosa rugosa)
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DOI：
10.2298/abs150310098f
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发表时间：
2015-01-01
影响因子：
0.8
通讯作者：
Sheng, Li-Xia
中科院分区：
文献类型：
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作者：
Feng, Li-Guo;Luan, Xiao-Fang;Sheng, Li-Xia
刺是一种由植物毛状体与少数皮层细胞一起变形而形成的尖状突起。它是一种形态特殊的多细胞无腺毛，起源于韧皮部，但不与木质部相连。玫瑰是一种重要的观赏/商业植物，也是食品和香料工业的重要原料。然而，其茎上的坚硬的刺给田间管理、花卉收获和花园管理带来不便。本研究从皱纹牡丹中分离到与皮刺发育相关的转录因子TTG1。对其在不同组织和品种中的表达模式进行了分析。结果表明，RrTTG1基因在叶片中的表达水平最高，茎次之，在果皮和花瓣中的表达水平较低。此外，与‘威海野月季’相比，RrTTG1基因在各组织中的表达水平均高于‘威海野月季’，这与田间形态观察结果一致。因此，RrTTG1转录因子可能调控玫瑰皮刺的发育。本研究为进一步探讨玫瑰花皮刺形成和发育的分子机制奠定了基础，为利用基因工程培育少刺或无刺玫瑰提供了分子基础。
A prickle is an acuminate protuberance formed by the deformation of plant trichomes together with a few cortical cells. It is a type of multicellular eglandular trichome with special morphology, which originates from the phloem but is not connected to the xylem. Rosa rugosa is an important ornamental/commercial plant and an important raw material in the food and perfume industries. However, the firm prickles on its stems are inconvenient to field management, the harvesting of flowers and garden management. The TTG1 transcription factor related to the development of prickle was isolated from R. rugosa in the present study. Its expression patterns in different tissues and varieties were analyzed. Results showed the expression level of the RrTTG1 gene was highest in the leaves, followed by the stems, but was lower in the pericarps and petals. Moreover, the higher expression level of the RrTTG1 gene in all tissues of the 'Ciguo rose', as compared with that of the 'Weihai wild rose', follows the results of field morphological observation. Therefore, the RrTTG1 transcription factor is likely to regulate the development of rose prickles. This study allows for further discussion on the molecular mechanisms of prickle formation and development in R. rugosa and provides a molecular basis for the cultivation of roses with fewer or no prickles via genetic engineering.