Mitochondrial COI and II provide useful markers for Wiseana (Lepidoptera: Hepialidae) species identification
Mitochondrial COI and II provide useful markers for Wiseana (Lepidoptera: Hepialidae) species identification
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DOI：
10.1017/s0007485399000437
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发表时间：
1999-08-01
影响因子：
1.9
通讯作者：
Paterson, AM
中科院分区：
文献类型：
--
作者：
Brown, B;Emberson, RM;Paterson, AM
描述了一种鉴定新西兰特有属Wiseana Viette成员的方法。该属共记述7种：W. cervinata（步行者），W. copularis（Meyrick），W. jocosa（Meyrick），宽叶草W. mimica（Philpott）、拟水虱W. signata（步行者）和W. umbraculata（Guenee）.没有形态特征已被确定，以区分每个物种的幼虫和成年女性表现出高水平的种内和种间形态变异，使识别困难或不可能。成年雄性可以通过鳞片、触角和生殖器特征的组合来区分，但这需要相当多的分类学经验。通过聚合酶链反应（PCR）扩增线粒体DNA的细胞色素氧化酶亚基I和II（COI和II）产生分子标记。用限制性内切酶消化扩增的DNA以得到特征性片段模式。14种限制性内切酶被调查，其中4种的组合区分了所有Wiseana类群，除了W。fuliglidae和W.拟态
A method for identifying the members of the endemic genus Wiseana Viette from New Zealand is described. Seven species have been described in the genus: W. cervinata (Walker), W. copularis (Meyrick), W, fuliginea (Butler), W. jocosa (Meyrick), W. mimica (Philpott), W. signata (Walker) and W. umbraculata (Guenee). No morphological characters have been identified to distinguish between the larvae of each species and adult females exhibit high levels of intra and interspecific morphological variation making identification difficult or impossible. Adult males can be distinguished by a combination of scale, antennal and genital characters, but this requires considerable taxonomic experience. Molecular markers were generated via amplification of the cytochrome oxidase subunit I and II (COI and II) of the mitochondrial DNA by the polymerase chain reaction (PCR). Amplified DNA was digested with restriction enzymes to give characteristic fragment patterns. Fourteen restriction enzymes were surveyed and a combination of four of these distinguish all Wiseana taxa except W. fuliginea and W. mimica.