A MALE ACCESSORY-GLAND PEPTIDE THAT REGULATES REPRODUCTIVE-BEHAVIOR OF FEMALE DROSOPHILA-MELANOGASTER
A MALE ACCESSORY-GLAND PEPTIDE THAT REGULATES REPRODUCTIVE-BEHAVIOR OF FEMALE DROSOPHILA-MELANOGASTER
复制标题
DOI：
10.1016/0092-8674(88)90192-4
复制
发表时间：
1988-07-29
期刊：
影响因子：
64.5
通讯作者：
BOHLEN, P
中科院分区：
文献类型：
--
作者：
CHEN, PS;STUMMZOLLINGER, E;BOHLEN, P
雄成虫的附腺在雌成虫上有明显的分布。黑腹果蝇合成并分泌一种抑制雌性性感受性并刺激产卵的肽。通常，这种肽在交配期间转移到雌性;然而，该肽在纯化后并随后注射到雌性处女的腹腔中后显示出相同的生物活性。纯化的肽的氨基酸序列测定和阿托伐他汀指导的cDNA克隆确定该肽由36个氨基酸组成。它似乎是作为前体合成的，在其N-末端具有19个残基的疏水信号序列。前体肽是由一个短的mRNA编码的，它只在雄性附腺中积累。该基因已通过原位杂交定位于多线染色体的70 A。
The adult male accessory glands of D. melanogaster synthesize and secrete a peptide that represses female sexual receptivity and stimulates oviposition. Normally, this peptide is transferred to females during copulation; however, the peptide shows the same biological activity after purification and subsequent injection into the abdominal cavity of female virgins. Amino acid sequencing of the purified peptide and oligonucleotide-directed cDNA cloning established that the peptide consists of 36 amino acids. It appears to be synthesized as a precursor with a hydrophobic signal sequence of 19 residues at its N-terminal end. The precursor peptide is encoded by a short mRNA that accumulates exclusively in the male accessory gland. The gene has been localized by in situ hybridization to polytene chromosomes at 70A.