Mitochondrial chromatin in Paramecium aurelia
Mitochondrial chromatin in Paramecium aurelia
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金黄色草履虫的线粒体染色质
DOI：
10.1007/bf00270499
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
A. Tait
中科院分区：
文献类型：
--
作者：
E. Olszewska;A. Tait
从草履虫线粒体中提取的物质中观察到染色质样结构，并提出证据证明这些结构不是来自线粒体制剂的核污染，而是完全来自线粒体。用稀酸提取这些染色质样结构，然后对提取物进行凝胶电泳，发现存在五种与组蛋白具有相似电泳迁移率的主要碱性蛋白。内源性核酸酶消化线粒体提取物，然后对提取的DNA进行电泳，证明线粒体基因组受到规则重复单元的保护，免受核酸酶消化，这与染色质核酸酶消化中观察到的非常相似。
Chromatin-like structures have been observed in material extracted from the mitochondria ofParamecium aureliaand evidence is presented which establishes that these structures do not originate from nuclear contamination of mitochondrial preparations but are exclusively of mitochondrial origin. Extraction of these chromatin-like structures with dilute acid followed by gel electrophoresis of the extract shows the presence of five major basic proteins which are of similar electrophoretic mobility to histones. Digestion of mitochondrial extracts with endogenous nuclease, followed by electrophoresis of the extracted DNA, demonstrated that the mitochondrial genome is protected from nuclease digestion by regular repeating units very similar to those observed in the nuclease digestion of chromatin.