Effects of glucocorticoids on the gene expression of nutrient transporters in different rabbit intestinal segments
Effects of glucocorticoids on the gene expression of nutrient transporters in different rabbit intestinal segments
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DOI：
10.1017/s1751731120000245
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发表时间：
2020-08-01
期刊：
影响因子：
3.6
通讯作者：
Li, F.
中科院分区：
文献类型：
--
作者：
Liu, H.;Liu, L.;Li, F.
Glucocorticoids (GCs) are counterregulatory hormones with broad effects on the digestion and absorption of dietary carbohydrates, lipids and proteins, but the underlying molecular mechanisms of these effects remain unclear. The present experiment was conducted to investigate the main expression sites of nutrient transporters and the effects of GCs on the gene expression of these transporters in the rabbit small intestine. The results showed that peptide transporter 1 (PepT1), facultative amino acid transporter (rBAT), neutral amino acid transporter (B(0)AT), excitatory amino acid transporter 3 (EAAT3), sodium-glucose transporter 1 (SGLT1) and glucose transporter 5 (GLUT5) were mainly expressed in the distal segment, glucose transporter 2 (GLUT2) and fatty-acid-binding protein 4 (FATP4) were mainly expressed in the proximal segment and cationic amino acid transporter 1 (CAT1) was mainly expressed in the middle segment of the rabbit small intestine. In addition, we analysed the effects of 3 h (short-term) or 7 days (long-term) dexamethasone (DEX) treatment on the gene expression of most nutrient transporters. The results showed that short-term DEX treatment significantly decreasedPepT1,B(0)AT,EAAT3,rBATandSGLT1expressions in all small intestinal segments, while it significantly decreasedGLUT2in the duodenum andFATP4in the duodenum and ileum (P< 0.05). Long-term DEX treatment also significantly decreasedPepT1,CAT1,B(0)AT,EAAT3,rBATandSGLT1in all small intestinal segments and significantly decreasedGLUT2in the jejunum andFATP4in the ileum (P< 0.05). In conclusion, DEX could decrease the gene expression of most nutrient transporters (exceptGLUT5) and affect the transport of intestinal amino acids, monosaccharides and fatty acids.
