Assessment of plant nitrogen status using chlorophyll fluorescence parameters of the upper leaves in winter wheat
Assessment of plant nitrogen status using chlorophyll fluorescence parameters of the upper leaves in winter wheat
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DOI：
10.1016/j.eja.2014.12.013
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发表时间：
2015-03-01
影响因子：
5.2
通讯作者：
Guo, Tian-Cai
中科院分区：
文献类型：
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作者：
Feng, Wei;He, Li;Guo, Tian-Cai
小麦氮素营养状况的无损、快速诊断对于小麦生长评价和氮素营养动态管理具有重要意义。两个小麦品种郑麦366（高蛋白含量）和矮抗58（中蛋白含量）在中国郑州连续两个生长季进行了五种不同氮素水平（0、90、180、270和360 kg ha（-1））的田间试验。同时测定了小麦不同生育期叶片叶绿素荧光（ChlF）参数、茎叶生物量和氮素含量，建立了基于ChlF参数的小麦氮素营养评价模型。结果表明，从返青期到开花期，顶3片叶（1-3LFT）的叶绿素参数差异较小。随着施氮水平的提高，第4叶（4LFT）与前3叶（1-3LFT）叶绿素参数的差异逐渐减小，表明4LFT对施氮敏感，在竞争氮素再分配方面处于劣势。上部完全展开叶片的叶绿素参数与相应叶片氮浓度的相关系数分别为：F-v = 0.628，F-m = 0.607，F-v/F-o = 0.579，F-v/F-m = 0.600。
Non-destructive, rapid diagnosis of plant nitrogen status is important for the evaluation of wheat growth and the dynamic management of nitrogen nutrition. Two wheat cultivars, Zhengmai 366 (high protein content) and Aikang 58 (medium protein content) were grown in field trials at five different nitrogen levels (0, 90, 180, 270 and 360 kg ha(-1)) in two consecutive growing seasons at Zhengzhou, China. Leaf chlorophyll fluorescence (ChlF) parameters, leaf and stem biomass, and nitrogen content were measured simultaneously at different growth stages, establishing an evaluation model of plant nitrogen nutrition in wheat using ChlF parameters. The results showed that the differences in ChlF parameters between the three top leaves (1-3LFT) was small from the reviving to the flowering stages. With increasing nitrogen levels, the difference in ChlF parameters between the fourth leaf (4LFT) and the first three leaves (1-3LFT) decreased gradually, indicating that 4LFT is sensitive to N fertilizer application and has a disadvantage in competition for redistributed N. The correlation coefficients between ChlF parameters for the upper, fully expanded leaves and N concentration of the corresponding leaves were 0.628 for F-v, 0.607 for F-m, 0.579 for F-v/F-o, and 0.600 for F-v/F-m at P