NEUROBLAST SPECIFICATION AND FORMATION REGULATED BY WINGLESS IN THE DROSOPHILA CNS
NEUROBLAST SPECIFICATION AND FORMATION REGULATED BY WINGLESS IN THE DROSOPHILA CNS
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DOI：
10.1126/science.8372355
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发表时间：
1993-09-17
期刊：
影响因子：
56.9
通讯作者：
DOE, CQ
中科院分区：
文献类型：
--
作者：
CHULAGRAFF, Q;DOE, CQ
果蝇中枢神经系统（CNS）由异质性的神经干细胞（成神经细胞）群体发育而来，但调控成神经细胞决定的基因仍不明确。分节基因无翅（wingless）在成神经细胞发育所源自的神经外胚层中呈区域性表达。利用一个条件性无翅突变来使中枢神经系统功能失活，同时不影响分节。在无翅失活后，表达无翅的神经外胚层条带产生看似正常的成神经细胞；然而，相邻的前部和后部神经外胚层在后续的成神经细胞决定和形成过程中，非自主性地需要无翅。无翅基因缺失导致已确定的成神经细胞缺失或重复，突显了它在中枢神经系统中产生成神经细胞多样性的作用。
The Drosophila central nervous system (CNS) develops from a heterogeneous population of neural stem cells (neuroblasts), yet the genes regulating neuroblast determination remain unknown. The segmentation gene wingless is regionally expressed in the neuroectoderm from which neuroblasts develop. A conditional wingless mutation is used to inactivate CNS function without affecting segmentation. The stripe of wingless-expressing neuroectoderm generates apparently normal neuroblasts after wingless inactivation; however, adjacent anterior and posterior neuroectoderm requires wingless nonautonomously for subsequent neuroblast determination and formation. Loss of wingless results in the absence or duplication of identified neuroblasts, highlighting its role in generating neuroblast diversity in the CNS.