Effects of early cleavage patterns of human embryos on subsequent in vitro development and implantation
Effects of early cleavage patterns of human embryos on subsequent in vitro development and implantation
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DOI：
10.1016/j.fertnstert.2016.04.020
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发表时间：
2016-08-01
影响因子：
6.7
通讯作者：
Ezono, Yuji
中科院分区：
文献类型：
--
作者：
Mizobe, Yamato;Oya, Naoto;Ezono, Yuji
目的：为了建立一个非侵入性的选择系统的人类胚胎具有高的植入潜力transfer.Design：队列研究。设置：独立的IVF诊所。患者（s）：164例患者，与791个胚胎受精，检查，并recorded.Intervention（s）：胚胎被分为9组，根据细胞的数量，他们包含和他们在第一次和第二次分裂的破碎程度。我们使用延时培养箱检测了胚胎的细胞分裂模式和分裂时间对体外和体内后续发育的影响。主要结果测量：囊胚形成率、高质量囊胚形成率和妊娠率。结果：胚胎发育成囊胚的比率以及内细胞团和滋养外胚层评分均为B或更高的囊胚的比率在第一次分裂期间形成两个细胞并且在第二次分裂期间形成四个细胞的组中，无论是否存在碎片化，高质量囊胚的发育率都很高。在这些组中，发育成优质囊胚的胚胎的第一次和第二次分裂时间（分别为25.90和37.88小时）显著短于其他胚胎。根据第一、二次分裂时间进行胚胎选择，虽然对培养后第2、3天的移植妊娠率无影响，但可提高培养后第5天的移植妊娠率。结论：培养后25.90、37.88h内完成第一、二次分裂的胚胎移植妊娠率高。(C)2016年，美国生殖医学会（American Society for Reproductive Medicine）
Objective: To establish a noninvasive selection system of human embryos with a high implantation potential before transferring.Design: Cohort study.Setting: Independent IVF clinics.Patient(s): One hundred sixty-four patients, with 791 embryos fertilized, checked, and recorded.Intervention(s): Embryos were divided into nine groups according to the number of cells they contained and the extent of their fragmentation during the first and second divisions. We examined the effects of the cell division pattern and division time of embryos on subsequent development in vitro and in vivo using a time-lapse incubator.Main Outcome Measure(s): Rates of blastocyst formation, high-quality blastocyst formation, and pregnancy.Result(s): The rates of embryos developed into blastocysts and blastocysts in which both the inner cell mass and trophectoderm grades were scored B or higher (good-quality blastocysts) were high in groups that formed two cells during the first division and four cells during the second division, regardless of the presence or absence of fragmentation. In these groups the first and second division times (25.90 and 37.88 hours after culture, respectively) of embryos developed into good-quality blastocysts were significantly shorter than those of other embryos. Although embryo selection based on the first and second division times did not affect the pregnancy rates after transfer at day 2 or 3 of culture, it improved the pregnancy rates after blastocyst transfer at day 5.Conclusion(s): Transfer of blastocysts derived from embryos that completed first and second divisions within 25.90 and 37.88 hours after culture, respectively, brings about high pregnancy rates. (C) 2016 by American Society for Reproductive Medicine.