The effects of growth factors on multicellular spheroids formed by chick embryonic retinal cells.
The effects of growth factors on multicellular spheroids formed by chick embryonic retinal cells.
复制标题
生长因子对鸡胚视网膜细胞形成的多细胞球体的影响。
DOI：
10.18926/amo/30788
复制
发表时间：
1997
影响因子：
0.5
通讯作者：
N. Matsuo
中科院分区：
文献类型：
--
作者：
T. Matsuo;M. Takabatake;N. Matsuo
将孕7.5天鸡胚视网膜细胞在非贴壁悬浮培养皿中培养两个月，研究生长因子及与视网膜色素上皮细胞共培养对其分化的影响。解离的视网膜细胞在一天内变成细胞聚集体（多细胞球体），并且在2天后在球体中形成视网膜细胞。超微结构上，神经元的roadly发达的连接纤毛，椭圆体（线粒体的积累），和外界膜，表明它们分化成感光细胞。表皮生长因子增强玫瑰花结形成神经元的视紫红质的表达，而碱性成纤维细胞生长因子在4周时诱导Mueller细胞的生长，并在8周时诱导其转分化为晶状体上皮样细胞。视网膜细胞与视网膜色素上皮细胞共培养可促进球状体中的类圆形细胞的形成。在培养皿中悬浮培养形成的多细胞球状体将为研究视网膜的分化和转分化提供一个方便的体外系统。
Retinal cells from chick embryos aged 7.5 days of gestation were cultured for two months in a non-adherent suspension culture dish to study the effects of growth factors and co-culture with retinal pigment epithelial cells on their differentiation. Dissociated retinal cells became cellular aggregates (multicellular spheroids) within a day, and rosettes were formed in the spheroids after 2 days. Ultrastructurally, neurons of the rosettes developed connecting cilia, ellipsoids (accumulation of mitochondria), and external limiting membrane, indicative of their differentiation into photoreceptor cells. Epidermal growth factor enhanced the expression of rhodopsin by rosette-forming neurons, while basic fibroblast growth factor induced the growth of Mueller cells at 4 weeks, and their transdifferentiation into lens-epithelial-like cells at 8 weeks. Co-culture of retinal cells with retinal pigment epithelial cells enhanced the formation of rosettes in spheroids. Multicellular spheroids formed in a dish for suspension culture would provide a convenient in vitro system to examine differentiation and transdifferentiation of the retina.