Overexpression of Dicer enhances RNAi-mediated gene silencing by short-hairpin RNAs (shRNAs) in human cells.
Overexpression of Dicer enhances RNAi-mediated gene silencing by short-hairpin RNAs (shRNAs) in human cells.
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DOI：
10.1093/nass/48.1.191
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发表时间：
2004-01-01
期刊：
影响因子：
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通讯作者：
Taira, Kazunari
中科院分区：
文献类型：
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作者：
Mikuma, Toshiyasu;Kawasaki, Hiroaki;Taira, Kazunari
RNA干扰(RNAi)是小干扰RNA(SiRNA)诱导序列依赖的mRNA降解的过程。近年来，几个小组开发了基于载体的siRNA表达系统。在这些系统中，短发夹状RNA(ShRNAs)被广泛选择作为第一种转录产物，这些产物通过依赖Dice的切割作用被加工成siRNAs。在这项研究中，我们证明了在哺乳动物细胞中，过表达DICER增强了依赖shRNA的基因沉默的效果。因此，这些结果表明依赖于DICER的shRNAs切割是shRNA介导的基因沉默的重要步骤。
RNA interference (RNAi) is a process in which small-interfering RNA (siRNA) induces a sequence-dependent degradation of mRNA. In recent years, vector-based siRNA expression systems have been developed by several groups. In these systems, short-hairpin RNAs (shRNAs) have been widely chosen for first transcriptional products which are processed to siRNAs by Dicer-dependent cleavage. In this study, we demonstrate that overexpression of Dicer enhances the effect of shRNA-dependent gene silencing in mammalian cells. Therefore, these results indicate that Dicer-dependent cleavage of shRNAs is an important step for shRNA-mediated gene silencing.