Cytotoxic effects of adriamycin on mouse hypoglossal neurons following retrograde axonal transport from the tongue
Cytotoxic effects of adriamycin on mouse hypoglossal neurons following retrograde axonal transport from the tongue
复制标题
阿霉素对小鼠舌头逆行轴突运输后舌下神经元的细胞毒性作用
DOI：
10.1007/bf00691980
复制
发表时间：
2004
影响因子：
12.7
通讯作者：
Y. Olsson
中科院分区：
文献类型：
--
作者：
L. Bigotte;Y. Olsson
摘要我们最近报道，荧光细胞抑制药物阿霉素（阿霉素）可能通过舌神经末梢逆行轴突转运到达舌下神经元。本研究旨在确定阿霉素逆行转运是否导致舌下神经元发生形态学变化。肌肉注射后14天，在舌下核中观察到神经元变性。将阿霉素注射到舌头中。早期的神经元变化，如核染色质稀疏、核仁成分分离和破碎，随后发生细胞质空泡化、核糖体消失和其他变性特征。从神经毒理学的角度来看，这些观察结果很重要，因为它们证明逆行轴突转运可能为阿霉素进入神经系统提供了一条途径。迄今为止，阿霉素似乎是唯一已知的可以直接在神经元中追踪并导致其变性的物质。介绍了一种破坏中枢神经系统运动神经元的实验方法。因此，使用阿霉素可以提供用于研究实验性运动神经元疾病的新毒性模型。
SummaryWe reported recently that the fluorescent, cytostatic drug, adriamycin (Doxorubicin) may reach the hypoglossal neurons by retrograde axonal transport from the nerve terminals of the tongue. The present investigation was undertaken to ascertain whether morphological changes occur in the hypoglossal neurons due to retrograde transport of adriamycin.Neuronal degeneration was observed in the hypoglossal nucleus 14 days after i.m. injection of adriamycin into the tongue. Early neuronal changes, such as rarefaction of the nuclear chromatin and segregation and fragmentation of the nucleolar components, were succeeded by cytoplasmic vacuolation, disappearance of ribosomes and other degenerative features.These observations are important from a neurotoxicologic viewpoint since they demonstrate that retrograde axonal transport may provide a route for the entry of adriamycin into the nervous system. Thus far, adriamycin appears to be the only known substance which can be traced directly in the neurons and cause their degeneration. An experimental method of damaging the motor neurons of the CNS has been introduced. A new toxic model for the investigation of experimental motor neuron disease is therefore available by the use of adriamycin.