Total cellular RNA content: correlation between flow cytometry and ultraviolet spectroscopy.
Total cellular RNA content: correlation between flow cytometry and ultraviolet spectroscopy.
复制标题
细胞总 RNA 含量：流式细胞术和紫外光谱之间的相关性。
DOI：
--
复制
发表时间：
1980
影响因子：
3.2
通讯作者：
L. Dethlefsen
中科院分区：
文献类型：
--
作者：
K. Bauer;L. Dethlefsen
Total RNA content in Chinese hamster ovary and HeLa-S3 cells determined by ultraviolet spectroscopy is compared with the red fluorescence distribution of acridine orange-stained cells observed by flow cytometry. A correlation coefficient of 0.93 is obtained when these methods of estimating RNA content are compared after various RNAse treatments. These data suggest that acridine orange staining effectively quantitates total cellular RNA content when analyzed by flow cytometry, although DNA is also shown to contribute a low but significant background of red fluorescence.
