Optimal fractionation and bioassay plans for isolation of synergistic chemicals: The subtractive-combination method
Optimal fractionation and bioassay plans for isolation of synergistic chemicals: The subtractive-combination method
复制标题
用于分离协同化学物质的最佳分馏和生物测定计划：减法组合法
DOI：
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发表时间：
1992
影响因子：
2.3
通讯作者：
J. Byers
中科院分区：
文献类型：
--
作者：
J. Byers
生物体的化学生态学的研究建立在化学信息素的分离和鉴定上，通常由两种或更多种协同化合物组成（每种协同剂单独具有很少的活性，但一起呈现时它们具有生物活性）。比较了二元分离法、加性组合法和减性组合法分离增效剂的效率。公式推导出后两种方法，计算预期数量的生物测定试验所需的隔离从两个到五个增效剂的生物提取物与任何数量的化合物，这取决于初始（主要）色谱馏分的数量。根据这些公式编制的计算机程序证明了减法组合法的优越性。与程序的模拟被用来确定最佳数量的初始馏分的加法和减法组合的方法时，从25至1200化合物的提取物中分离出两至五个增效剂。讨论了生物测定、分离、鉴定和田间试验的方法。
Studies of chemical ecology of an organism are founded on the isolation and identification of a semiochemical, often comprised of two or more synergistic compounds (each Synergist alone has little activity, but presented together they are bioactive). Chromatographie fractionation and bioassay methods of binary splitting, additive combination, and subtractive combination are compared for efficiency in isolating synergists. Formulas are derived for the latter two methods that calculate the expected number of bioassay tests required for isolation of from two to five synergists from biological extracts with any number of compounds, depending on the number of initial (major) Chromatographic fractions. A computer program based on the formulas demonstrates the superiority of the subtractive-combination method. Simulations with the program were used to determine the optimal number of initial fractions for the additive- and subtractive-combination methods when isolating two to five synergists from extracts of from 25 to 1200 compounds. Methods of bioassay, isolation, identification, and field testing of semiochemicals are discussed.