Morphological and Stereological Characterization of Hepatic Foci of Cellular Alteration in Control Fischer 344 Rats∗
Morphological and Stereological Characterization of Hepatic Foci of Cellular Alteration in Control Fischer 344 Rats∗
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对照 Fischer 344 大鼠肝细胞改变灶的形态学和体视学特征*
DOI：
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
G. Boorman
中科院分区：
文献类型：
--
作者：
T. Harada;R. Maronpot;Richard W. Morris;K. Stitzel;G. Boorman
对来自国家毒理学计划（NTP）进行的7项2年致癌性研究的两种性别对照Fischer 344（F344）大鼠的标准苏木精和伊红染色肝脏切片进行了肝细胞病灶改变（AHF）的定量评价，随后进行了体视学分析。在研究第6、9、12、15、18和/或24个月时采集肝脏样本。虽然AHF具有广泛的形态学特征，但根据先前发表的标准，可将其分为以下5种类型：嗜碱性灶、嗜酸性灶、透明灶、空泡灶和混合细胞灶。约50%的动物在6个月时有病灶，15个月时两种性别的发病率接近100%。AHF的数量，大小和体积分数随着年龄的增加，在两种性别，这些变化是最明显的嗜碱性和透明细胞灶。雌性动物的嗜碱性病灶数量显著多于雄性动物，而雄性动物的透明细胞病灶数量更多。在每个时间点均观察到这种性别差异。24个月时，雄性和雌性动物中所有类型AHF的平均数量分别为547和460/立方厘米肝脏。尽管对照组大鼠的AHF发生率较高，但肝细胞肿瘤的发生率较低。这意味着在2年研究中使用的对照F344大鼠中，大多数病灶不会进展为瘤形成。
Quantitative evaluation of altered hepatocellular foci (AHF), followed by stereological analysis was performed on standard hematoxylin and eosin-stained liver sections from control Fischer 344 (F344) rats of both sexes from seven 2-yr carcinogenicity studies conducted by the National Toxicology Program (NTP). Liver samples were collected at 6, 9, 12, 15, 18, and/or 24 months on study. Although AHF had a broad spectrum of morphological features, they could be classified into the following 5 types using previously published criteria: basophilic, eosinophilic, clear, vacuolated, and mixed cell foci. Approximately 50% of the animals had foci at 6 months, and the incidence reached nearly 100% at 15 months in both sexes. The number, size and volume fraction of AHF increased with age in both sexes; these changes were most evident for basophilic and clear cell foci. The number of basophilic foci was significantly greater in females than in males while clear cell foci were more numerous in males. This sex difference was observed at each time point. Mean number of all types of AHF in males and females at 24 months was 547 and 460 per cubic centimeter of liver, respectively. Despite the high incidence of AHF in control rats, the incidence of hepatocellular neoplasms is low. The implication is that most foci do not progress to neoplasia in control F344 rats used in 2-yr studies.
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苯巴比妥和二乙基亚硝胺给药后大鼠肝脏酶改变病灶的自然史和剂量反应特征。
影响因子：
4.7
作者：
Goldsworthy,T;Campbell,HA;Pitot,HC
通讯作者：
Pitot,HC
使用 γ-谷氨酰转肽酶、乙醛脱氢酶、葡萄糖-6-磷酸脱氢酶和甲基丙烯酸酯包埋肝脏中的其他标记物检查酶改变的病灶。
DOI：
--
发表时间：
1987
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Pretlow,TP;Grane,RW;Goehring,PL;Lapinsky,AS;Pretlow2nd,TG
通讯作者：
Pretlow2nd,TG
定量体视学在评估大鼠肝脏酶改变病灶中的应用。
影响因子：
11.2
作者：
Campbell,HA;Pitot,HC;Potter,VR;Laishes,BA
通讯作者：
Laishes,BA
在二乙基亚硝胺给药和苯巴比妥促进后，大鼠肝脏改变的肝病灶的组织化学标记物的定量分析和稳定性。
影响因子：
4.7
作者：
Goldsworthy,TL;Pitot,HC
通讯作者：
Pitot,HC
用于估计横切面的微观肝细胞病灶数量的可靠体视学方法。
影响因子：
11.2
作者：
Pugh,TD;King,JH;Koen,H;Nychka,D;Chover,J;Wahba,G;He,YH;Goldfarb,S
通讯作者：
Goldfarb,S