Reversal effect of vitamin D on different multidrug-resistant cells.
Reversal effect of vitamin D on different multidrug-resistant cells.
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维生素 D 对不同多重耐药细胞的逆转作用。
DOI：
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发表时间：
2014
影响因子：
0.4
通讯作者：
H. Nuriding
中科院分区：
文献类型：
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作者：
M. Yan;H. Nuriding
我们研究了维生素D对多重耐药白血病Jurkat/ADR和K562/ADR细胞系的逆转作用，并对其逆转机制进行了初步探讨。采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑(MTT)法检测维生素D对多重耐药细胞的逆转作用。采用实时聚合酶链反应测定维生素D对细胞内多重耐药基因（MDRI）和多重耐药相关基因（MRP1）mRNA表达的影响。采用蛋白质定量分析方法测定维生素D对细胞内谷胱甘肽含量的影响。用维生素D处理Jurkat/ADR和K562/ADR细胞后，多药耐药性以剂量依赖性方式逆转，这可能降低了MDR1和MRP1基因的mRNA表达、细胞表面的P-糖蛋白含量和细胞内谷胱甘肽水平。不同浓度的维生素D对不同的多重耐药细胞表现出不同的逆转作用。其耐药机制可能与抑制MDR1和MRP1基因的表达有关。
We investigated the reversal effect of vitamin D on the multidrug-resistant leukemic Jurkat/ADR and K562/ADR cell lines and conducted a preliminary investigation of its reversal mechanism. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method was used to detect the reversal effect of vitamin D on multidrug-resistant cells. Real-time polymerase chain reaction was used to determine the effect of vitamin D on intracellular expression of mRNA of the multidrug-resistant gene (MDRI) and the multidrug-resistance-related gene (MRP1). A protein quantitative analysis method was used to determine the effect of vitamin D on intracellular glutathione content. After treatment of Jurkat/ADR and K562/ADR cells with vitamin D, multidrug resistance was reversed in a dose-dependent manner, which may have reduced mRNA expression of the MDR1 and MRP1 genes, the P-glycoprotein content on the cell surface, and the intracellular glutathione level. Different concentrations of vitamin D showed varying reversal effects on different multidrug-resistant cells. The resistance mechanism may be related to the inhibition of the expression of MDR1 and MRP1 genes.
影响因子：
3.9
作者：
Welsh, JoEllen
通讯作者：
Welsh, JoEllen
影响因子：
20.3
作者：
Moghrabi, Albert;Levy, Donna E.;Silverman, Lewis B.
通讯作者：
Silverman, Lewis B.