An NAD(P)H-nicotine blue oxidoreductase is part of the nicotine regulon and may protect Arthrobacter nicotinovorans from oxidative stress during nicotine catabolism
An NAD(P)H-nicotine blue oxidoreductase is part of the nicotine regulon and may protect Arthrobacter nicotinovorans from oxidative stress during nicotine catabolism
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DOI：
10.1128/aem.02668-06
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发表时间：
2007-04-01
影响因子：
4.4
通讯作者：
Brandsch, Roderich
中科院分区：
文献类型：
--
作者：
Mihasan, Marius;Chiribau, Calin-Bogdan;Brandsch, Roderich
NAD(P)H-烟碱蓝(苯醌)氧化还原酶是烟草节杆菌尼古丁分解代谢途径的一员。转录分析和电子迁移率改变分析表明，该酶基因在转录激活剂PmfR的调控下以尼古丁依赖的方式表达，是烟碱调节基因的一部分。含有黄素单核苷酸的酶使用NADH和效率较低的NADPH，通过两电子转移将尼古丁蓝还原为尼古丁蓝亮氨酸形式(对苯二酚)。除了尼古丁蓝，这种酶还能还原其他几种对苯二酚。NAD(P)H-尼古丁蓝氧化还原酶可能阻止细胞内尼古丁蓝的单电子还原，这可能导致半醌自由基和潜在的有毒活性氧物种。
An NAD(P)H-nicotine blue (quinone) oxidoreductase was discovered as a member of the nicotine catabolic pathway of Arthrobacter nicotinovorans. Transcriptional analysis and electromobility shift assays showed that the enzyme gene was expressed in a nicotine-dependent manner under the control of the transcriptional activator PmfR and thus was part of the nicotine regulon of A. nicotinovorans. The flavin mononucleotide-containing enzyme uses NADH and, with lower efficiency, NADPH to reduce, by a two-electron transfer, nicotine blue to the nicotine blue leuco form (hydroquinone). Besides nicotine blue, several other quinones were reduced by the enzyme. The NAD(P)H-nicotine blue oxidoreductase may prevent intracellular one-electron reductions of nicotine blue which may lead to semiquinone radicals and potentially toxic reactive oxygen species.