Quantification of ethanol methyl 1H magnetic resonance signal intensity following intravenous ethanol administration in primate brain
Quantification of ethanol methyl 1H magnetic resonance signal intensity following intravenous ethanol administration in primate brain
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DOI：
10.1016/j.ymeth.2009.12.004
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发表时间：
2010-03-01
期刊：
影响因子：
4.8
通讯作者：
Kroenke, Christopher D.
中科院分区：
文献类型：
--
作者：
Flory, Graham S.;O'Malley, Jean;Kroenke, Christopher D.
体内H-1磁共振波谱（MRS）可用于直接监测脑乙醇。此前，对人类受试者的研究表明，乙醇甲基H-1 MRS信号强度与对乙醇中毒作用的耐受性有关。最近，由于这些环境中T-2的差异，乙醇H-1 MRS信号强度在脑灰质（GM）、白质（WM）和脑脊液（CSF）之间存在差异。本文提出的方法将乙醇磁共振成像技术扩展到非人灵长类动物。12只猴子在镇静状态下注射乙醇，并放置在3T MRI系统中。静脉输注1 g/kg乙醇后进行化学位移成像（CSI）测量。还记录了每只猴子的磁共振成像（MRI）数据，以提供每个CSI谱的GM、WM和CSF的体积分数。为了估计乙醇MRS强度与GM、WM和CSF体积分数的协方差，在校正了射频脉冲谱不均匀性、化学偏移伪影以及CSI数据和成像数据中点扩散函数之间的差异后，确定了每种组织亚型的相对贡献。在GM、WM和CSF之间，发现单位血乙醇浓度的乙醇MRS强度存在差异。GM组MRS强度个体差异大于WM组。该方法证明了乙醇MRS实验和分析在非人类灵长类动物中的可行性，并表明转基因可能是个体间乙醇MRS强度显著差异的一个位点。(C) 2009爱思唯尔公司版权所有。
In vivo H-1 magnetic resonance spectroscopy (MRS) can be used to directly monitor brain ethanol. Previously, studies Of human subjects have lead to the suggestion that the ethanol methyl H-1 MRS signal intensity relates to tolerance to ethanol's intoxicating effects. More recently, the ethanol H-1 MRS signal intensity has been recognized to vary between brain gray matter (GM), white matter (WM), and cerebrospinal fluid (CSF) due to differences in T-2 within these environments. The methods presented here extend ethanol MRS techniques to non-human primate subjects. Twelve monkeys were administered ethanol while sedated and positioned within a 3T MRI system. Chemical shift imaging (CSI) measurements were performed following intravenous infusion of 1 g/kg ethanol. Magnetic resonance imaging (MRI) data were also recorded for each monkey to provide volume fractions of GM, WM, and CSF for each CSI spectrum. To estimate co-variance of ethanol MRS intensity with GM, WM, and CSF volume fractions, the relative contribution of each tissue subtype was determined following corrections for radiofrequency pulse profile non-uniformity, chemical shift artifacts, and differences between the point spread function in the CSI data and the imaging data. The ethanol MRS intensity per unit blood ethanol concentration was found to differ between GM, WM, and CSF. Individual differences in MRS intensity Were larger in GM than WM. This methodology demonstrates the feasibility of ethanol MRS experiments and analysis in non-human primate subjects, and suggests GM may be a site of significant variation in ethanol MRS intensity between individuals. (C) 2009 Elsevier Inc. All rights reserved.