Establishment of canine RNA polymerase I-driven reverse genetics for influenza a virus: Its application for H5N1 vaccine production
Establishment of canine RNA polymerase I-driven reverse genetics for influenza a virus: Its application for H5N1 vaccine production
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DOI：
10.1128/jvi.01876-07
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发表时间：
2008-02-01
影响因子：
5.4
通讯作者：
Kawaoka, Yoshihiro
中科院分区：
文献类型：
--
作者：
Murakami, Shin;Horimoto, Taisuke;Kawaoka, Yoshihiro
在发生新的流感大流行时，必须迅速生产抗原性与流行毒株相匹配的疫苗。在这里，我们使用犬多聚酶I(Poll)启动子序列建立了一种替代的流感病毒反向遗传学系统，该系统在Madin-Darby犬肾细胞系中有效工作，该细胞系被批准用于人类疫苗生产。使用这个系统，我们能够比目前在非洲绿猴Vero细胞中使用人类Poli启动子的系统更有效地产生H5N1疫苗种子病毒，从而提高大流行疫苗的生产。
In the event of a new influenza pandemic, vaccines whose antigenicities match those of circulating strains must be rapidly produced. Here, we established an alternative reverse genetics system for influenza virus using the canine polyrnerase I (Poll) promoter sequence that works efficiently in the Madin-Darby canine kidney cell line, a cell line approved for human vaccine production. Using this system, we were able to generate H5N1 vaccine seed viruses more efficiently than can be achieved with the current system that uses the human PolI promoter in African green monkey Vero cells, thus improving pandemic vaccine production.