CLASSIFICATION OF IMAGES OF BIOMOLECULAR ASSEMBLIES - A STUDY OF RIBOSOMES AND RIBOSOMAL-SUBUNITS OF ESCHERICHIA-COLI
CLASSIFICATION OF IMAGES OF BIOMOLECULAR ASSEMBLIES - A STUDY OF RIBOSOMES AND RIBOSOMAL-SUBUNITS OF ESCHERICHIA-COLI
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DOI：
10.1111/j.1365-2818.1988.tb04602.x
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发表时间：
1988-05-01
影响因子：
2
通讯作者：
WAGENKNECHT, T
中科院分区：
文献类型：
--
作者：
FRANK, J;BRETAUDIERE, JP;WAGENKNECHT, T
对在电子显微镜中获得的大分子图像进行对应分析。在所得到的低维因子空间中的数据中固有的结构的特征在于通过混合分类方法，该方法将动态云聚类技术与分层上升分类（HAC）相结合。对于我们的数据，动态云聚类获得的边缘集群的拒绝出现作为一个稳定的性能HAC的一个重要先决条件。该方法被应用到两组204和177的图像，显示了大肠杆菌的70 S核糖体，在由A. Verschoor和同事，以及两组分别为480和496的E.在明确的冠状视图中，L7/L12蛋白缺失的大肠杆菌。可再现的类，其特征在于从阶乘坐标重构的图像。这些类似乎是有关的试样网格上的不同取向（在70 S颗粒的情况下）和不同的构象状态（50 S亚基）。
Images of macromolecules obtained in the electron microscope are subjected to correspondence analysis. The structure inherent in the data in the resulting low-dimensional factor space is characterized by a mixed classification method which combines the dynamic clouds clustering technique with hierarchical ascendant classification (HAC). For our data, the rejection of marginal clusters obtained by dynamic clouds clustering appears as a crucial prerequisite for a stable performance of HAC. The method is applied to two sets of 204 and 177 images that show the 70S ribosome of Escherichia coli, in the range of overlap views as defined by A. Verschoor and co-workers, and to two sets of 480 and 496 images of the 50S subunit of E. coli depleted of L7/L12 proteins in the well-defined crown view. Reproducible classes are obtained, which are characterized by images reconstituted from factorial coordinates. These classes appear to be related to different orientations on the specimen grid (in the case of the 70S particle) and to different conformational states (50S subunit).