Automated solid phase extraction and quantitative analysis of human milk for 13 phthalate metabolites
Automated solid phase extraction and quantitative analysis of human milk for 13 phthalate metabolites
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DOI：
10.1016/j.jchromb.2004.02.006
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发表时间：
2004-06-05
影响因子：
3
通讯作者：
Needham, LL
中科院分区：
文献类型：
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作者：
Calafat, AM;Slakman, AR;Needham, LL
虽然母乳喂养的好处已得到广泛认可，但母乳是暴露于环境化学品的一种可能途径。包括邻苯二甲酸盐。因为邻苯二甲酸盐对哺乳期儿童的潜在健康影响。确定母乳中是否存在邻苯二甲酸盐是重要的。我们开发了一种灵敏的方法来测量13邻苯二甲酸酯代谢物在母乳中使用自动固相萃取（SPE）耦合同位素稀释-高效液相色谱（HPLC）-负离子电喷雾电离-串联质谱。我们使用D-4-邻苯二甲酸二酯来明确地确定人类母乳中存在能够将普遍存在的邻苯二甲酸二酯水解为它们各自的单酯的酶。该分析方法包括在采集乳汁后对酶进行酸变性，以避免采样期间引入的污染物邻苯二甲酸二酯水解。存储.和分析该方法具有良好的重现性（平均变异系数在4 ~ 27%之间）和准确度（加标回收率接近100%）。在1 ml母乳中的检测限为低ng/ml。我们在合并的人类母乳样本中检测到几种邻苯二甲酸酯代谢物，这表明邻苯二甲酸酯可以掺入母乳并转移到哺乳期儿童。(C)2004 Elsevier B. V.保留所有权利。
While the demonstrated benefits associated with breastfeeding are well recognized, breast milk is one possible route of exposure to environmental chemicals. including phthalates, by breastfeeding infants. Because of the potential health impact of phthalates to nursing children. determining whether phthalates are present in breast milk is important. We developed a sensitive method for measuring 13 phthalate metabolites in breast milk using automated solid phase extraction (SPE) coupled to isotope dilution-high-performance liquid chromatography (HPLC)-negative ion electrospray ionization-tandem mass spectrometry. We used D-4-phthalate diesters to unequivocally establish the presence in human breast milk of enzymes capable of hydrolyzing the ubiquitous phthalate diesters to their respective monoesters. The analytical method involves acid-denaturation of the enzymes after collection of the milk to avoid hydrolysis of contaminant phthalate diesters introduced during sampling. storage. and analysis. The method shows good reproducibility (average coefficient of variations range between 4 and 27%) and accuracy (spiked recoveries are similar to100%). The detection limits are in the low ng/ml range in 1 ml of breast milk. We detected several phthalate metabolites in pooled human breast milk samples, suggesting that phthalates can be incorporated into breast milk and transferred to the nursing child. (C) 2004 Elsevier B.V. All rights reserved.