Metabolic cycling in single yeast cells from unsynchronized steady-state populations limited on glucose or phosphate
Metabolic cycling in single yeast cells from unsynchronized steady-state populations limited on glucose or phosphate
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DOI：
10.1073/pnas.1002422107
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发表时间：
2010-04-13
影响因子：
11.1
通讯作者：
Botstein, David
中科院分区：
文献类型：
--
作者：
Silverman, Sanford J.;Petti, Allegra A.;Botstein, David
大部分酵母基因表达模式的振荡与“代谢循环”有关，通常只在预饥饿的连续酵母培养物中可见。我们使用单个细胞中mRNA的FISH来检验这些振荡发生在从恒化器中呈指数增长的非同步培养物中提取的单个细胞中的假设。来自同步化培养物的基因表达数据用于预测来自非同步化细胞中的基因对的mRNA的同时出现。FISH结果的定量分析表明，由于葡萄糖限制或磷酸盐限制，个别不同步的细胞生长缓慢，显示出预测的振荡。我们的结论是，酵母代谢循环是酵母代谢的内在属性，并不依赖于同步或外部限制生长的碳源。
Oscillations in patterns of expression of a large fraction of yeast genes are associated with the "metabolic cycle," usually seen only in prestarved, continuous cultures of yeast. We used FISH of mRNA in individual cells to test the hypothesis that these oscillations happen in single cells drawn from unsynchronized cultures growing exponentially in chemostats. Gene-expression data from synchronized cultures were used to predict coincident appearance of mRNAs from pairs of genes in the unsynchronized cells. Quantitative analysis of the FISH results shows that individual unsynchronized cells growing slowly because of glucose limitation or phosphate limitation show the predicted oscillations. We conclude that the yeast metabolic cycle is an intrinsic property of yeast metabolism and does not depend on either synchronization or external limitation of growth by the carbon source.