Analysis of IL1 gene polymorphisms and transcript levels in periodontal and chronic kidney disease
Analysis of IL1 gene polymorphisms and transcript levels in periodontal and chronic kidney disease
复制标题
DOI：
10.1016/j.cyto.2012.06.006
复制
发表时间：
2012-10-01
期刊：
影响因子：
3.8
通讯作者：
Trevilatto, Paula Cristina
中科院分区：
文献类型：
--
作者：
Ribeiro Braosi, Ana Paula;de Souza, Cleber Machado;Trevilatto, Paula Cristina
慢性肾病（CKD）和牙周炎（PD）是复杂的炎症紊乱，受遗传因素影响。白细胞介素 (IL)-1 基因编码参与这两种疾病的生理发病机制的炎症介质。 ILA 基因的功能多态性可调节细胞因子水平，并与免疫炎症性疾病的易感性相关。 目的：本研究的目的是探讨功能性 IL1 基因多态性和转录水平与 CKD 和 PD 易感性之间的关系。设计：样本由 246 名个体组成，平均年龄 44.8 岁，分为：第 1 组（64 名无 CKD 和无 PD 患者）、第 2 组（58 名无 CKD 和 PD 患者）、第 3 组（52 名 CKD 和无 PD 患者）和第 4 组（72 名 CKD 和 PD 患者）。从口腔粘膜细胞获得DNA，并通过PCR-RFLP分析ILIAC-889T、/L1BC-511T、IL1BC+3954T和IL1RN(内含子2)的多态性。通过实时 PCR 分析牙龈组织的转录水平。结果：IL1RN*1 等位基因与 CKD 风险增加近 4 倍相关（OR 3.92 95% CI = 1.6-9.4，p = 0.002）。 1L1RN*2 等位基因与 CKD 患者 PD 风险增加 3 倍相关（OR 3.08 95% CI = 1.2-7.9，p = 0.019）。 IL1B+3954 多态性的等位基因 T 与 PD 患者的 CKD 相关（OR 2.28 95% CI = 1.1-4.7，p = 0.019）。 PD 患者中 ILIA、IL1B 和 !URN 基因的转录水平显着升高。结论：观察到 IL1B 和 IL1RN 等位基因与 CKD 和 PD 易感性相关的证据。在 PD 患者中发现了较高水平的 IL1 基因转录本。 (C) 2012 Elsevier Ltd. 保留所有权利。
Chronic kidney disease (CKD) and periodontitis (PD) are complex inflammatory disturbances, influenced by genetic factors. Interleukin (IL)-1 genes code for inflammatory mediators involved in the physiopathogenesis of both diseases. Functional polymorphisms in ILA genes modulate cytokine levels and have been associated with susceptibility to immune-inflammatory conditions.Objectives: The aim of this study was investigate the association of functional IL1 gene polymorphisms and transcript levels with susceptibility to CKD and PD. Design: The sample consisted of 246 individuals, mean age 44.8 years, divided into: group 1 (64 patients without CKD and without PD), group 2 (58 without CKD and with PD), group 3 (52 with CKD and without PD) and group 4 (72 with CKD and with PD). DNA was obtained from cells of oral mucosa and polymorphisms ILIAC-889T, /L1BC-511T, IL1BC+3954T and IL1RN (intron 2) were analyzed by PCR-RFLP. Transcript levels from gingival tissues were analyzed by real-time PCR.Results: IL1RN*1 allele was associated with almost 4-fold increased risk for CKD (OR 3.92 95% CI = 1.6-9.4, p = 0.002). 1L1RN*2 allele was associated with 3-fold increased risk for PD in CKD patients (OR 3.08 95% CI = 1.2-7.9, p = 0.019). Allele T for polymorphism IL1B+3954 was associated with CKD in PD patients (OR 2.28 95% CI = 1.1-4.7,p = 0.019). Significantly increased levels of transcripts of ILIA, IL1B and !URN genes were found in PD patients.Conclusions: It was observed an evidence for association of IL1B and IL1RN alleles with susceptibility to CKD and PD. Higher levels of IL1 gene transcripts were found in PD patients. (C) 2012 Elsevier Ltd. All rights reserved.