Intestinal bacterial communities that produce active estrogen-like compounds enterodiol and enterolactone in humans
Intestinal bacterial communities that produce active estrogen-like compounds enterodiol and enterolactone in humans
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DOI：
10.1128/aem.71.10.6077-6085.2005
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发表时间：
2005-10-01
影响因子：
4.4
通讯作者：
Blaut, M
中科院分区：
文献类型：
--
作者：
Clavel, T;Henderson, G;Blaut, M
木脂素是膳食中的二酚类化合物，需要肠道细菌活化才能发挥可能的有益健康作用。肠道生态系统在木脂素代谢中起着至关重要的作用，但所涉及的生物体描述甚少。为了表征负责开环异落叶松树脂酚（SECO）活化的细菌群落，即，产生肠木脂素肠二醇（艾德）和肠内酯（EL）的群落，对24名人类受试者进行了研究。在所有测试的粪便样品中检测到SECO活化。最大可能数计数显示，参与艾德产生的肠道细菌是优势微生物群的一部分（6 x 10（8）CFU g（-1））。相反，催化EL形成的微生物的平均浓度约为3 × 10（5）CFU g（-1）。女性体内产生艾德和EL的微生物浓度往往高于男性。显着更大量的EL产生的粪便稀释液从个人与中到高浓度的EL生产细菌。从人粪便中分离出两种能够使SECO脱甲基和脱羟基的微生物。根据16 S rRNA基因序列分析，将这两株菌分别命名为Peptostreenoproductus SECO-Mt 75 m3和Eggerthella lenta SECO-Mt 75 m2。设计了一种新的16 S rRNA靶向寡核苷酸探针，该探针对P. productus和相关物种具有特异性，并与另外五种组特异性探针一起沿着进一步用于荧光原位杂交实验。显著较高比例的P. productus和相关物种（P = 0.012），以及属于Atopobium组（P = 0.035）的细菌，是典型的具有中到高浓度的EL生产社区的个体。
Lignans are dietary diphenolic compounds which require activation by intestinal bacteria to exert possible beneficial health effects. The intestinal ecosystem plays a crucial role in lignan metabolism, but the organisms involved are poorly described. To characterize the bacterial communities responsible for secoisolariciresinol (SECO) activation, i.e., the communities that produce the enterolignans enterodiol (ED) and enterolactone (EL), a study with 24 human subjects was undertaken. SECO activation was detected in all tested fecal samples. The intestinal bacteria involved in ED production were part of the dominant microbiota (6 x 10(8) CFU g(-1)), as revealed by most-probable-number enumerations. Conversely, organisms that catalyzed the formation of EL occurred at a mean concentration of approximately 3 x 10(5) CFU g(-1). Women tended to have higher concentrations of both ED- and EL-producing organisms than men. Significantly larger amounts of EL were produced by fecal dilutions from individuals with moderate to high concentrations of EL-producing bacteria. Two organisms able to demethylate and dehydroxylate SECO were isolated from human feces. Based on 16S rRNA gene sequence analyses, they were named Peptostreptococcus productus SECO-Mt75m3 and Eggerthella lenta SECO-Mt75m2. A new 16S rRNA-targeted oligonucleotide probe specific for P. productus and related species was designed and further used in fluorescent in situ hybridization experiments, along with five additional group-specific probes. Significantly higher proportions of P. productus and related species (P = 0.012), as well as bacteria belonging to the Atopobium group (P = 0.035), were typical of individuals with moderate to high concentrations of EL-producing communities.