Protein kinase Cη activates NF-κB in response to camptothecin-induced DNA damage
Protein kinase Cη activates NF-κB in response to camptothecin-induced DNA damage
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DOI：
10.1016/j.bbrc.2011.07.090
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发表时间：
2011-08-26
影响因子：
3.1
通讯作者：
Livneh, Etta
中科院分区：
文献类型：
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作者：
Raveh-Amit, Hadas;Hai, Naama;Livneh, Etta
核因子κ B（NF-κ B）家族的转录因子参与先天性和适应性免疫应答、细胞死亡和炎症相关基因的调节。蛋白激酶C（PKC）家族参与NF-κ B B在炎症和免疫相关信号传导中的调节已被广泛研究。然而，对于PKC在NF-κ B B调控中的作用，目前还知之甚少。在这里，我们首次证明了PKC-eta（PKC eta）通过激活I κ B激酶（IKK）和I κ B的降解来调节NF-κ B上游信号传导。此外，在非应激条件下和对抗癌药物喜树碱的应答中，PKC eta增强核转位和NF-κ B的反式激活。我们和其他人以前已经表明，PKC eta赋予对DNA损伤诱导的细胞凋亡的保护。我们目前的研究表明，PKC eta参与NF-κ B B信号传导，导致耐药。(C)2011 Elsevier Inc. All rights reserved.
The nuclear factor kappa B (NF-kappa B) family of transcription factors participates in the regulation of genes involved in innate- and adaptive-immune responses, cell death and inflammation. The involvement of the Protein Kinase C (PKC) family in the regulation of NF-kappa B in inflammation and immune-related signaling has been extensively studied. However, not much is known on the role of PKC in NF-kappa B regulation in response to DNA damage. Here we demonstrate for the first time that PKC-eta (PKC eta) regulates NF-kappa B upstream signaling by activating the I kappa B kinase (IKK) and the degradation of I kappa B. Furthermore, PKC eta enhances the nuclear translocation and transactivation of NF-kappa B under non-stressed conditions and in response to the anticancer drug camptothecin. We and others have previously shown that PKC eta confers protection against DNA damage-induced apoptosis. Our present study suggests that PKC eta is involved in NF-kappa B signaling leading to drug resistance. (C) 2011 Elsevier Inc. All rights reserved.