Development of persistent gastrointestinal S. aureus carriage in mice.
Development of persistent gastrointestinal S. aureus carriage in mice.
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DOI：
10.1038/s41598-017-12576-0
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发表时间：
2017-09-29
影响因子：
4.6
通讯作者：
Wyllie DH
中科院分区：
文献类型：
--
作者：
Flaxman A;van Diemen PM;Yamaguchi Y;Allen E;Lindemann C;Rollier CS;Milicic A;Wyllie DH
五分之一至四分之一的人口无症状地、自然地和持续地被金黄色葡萄球菌定植。观察性人体研究表明，尽管整个人群间歇性暴露，但一些个体迅速失去金黄色葡萄球菌。根据鼻腔培养的评估，其他的则成为持久的携带者，许多人在那里定居了几十年。目前金黄色葡萄球菌定植的动物模型价格昂贵，通常需要抗生素。重要的是，这些动物模型尚未有助于我们对人类殖民状态二分法的理解。在这里，我们鉴定了一株被发现持续定植在BALB/c小鼠胃肠道的金黄色葡萄球菌。系统发育分析表明，它在最近的过去从人类ST15谱系中分化出来。我们的研究表明，这种有机体在小鼠的肠道和鼻腔中发生，在生命早期获得，并且可以持续数月。重要的是，我们在基因相同的小鼠中观察到持续性和非持续性胃肠道运输状态的发展。我们开发了一个无针和无抗生素的模型，在这个模型中，我们很容易通过环境暴露诱导金黄色葡萄球菌在胃肠道的定植。利用我们的实验模型，可以评估适应性免疫对金黄色葡萄球菌定殖的影响。利用该模型也可以研究疫苗消除定植的功效。
One fifth to one quarter of the human population is asymptomatically, naturally and persistently colonised by Staphylococcus aureus. Observational human studies indicate that although the whole population is intermittently exposed, some individuals lose S. aureus rapidly. Others become persistent carriers, as assessed by nasal cultures, with many individuals colonised for decades. Current animal models of S. aureus colonisation are expensive and normally require antibiotics. Importantly, these animal models have not yet contributed to our poor understanding of the dichotomy in human colonisation status. Here, we identify a single strain of S. aureus found to be persistently colonising the gastrointestinal tract of BALB/c mice. Phylogenetic analyses suggest it diverged from a human ST15 lineage in the recent past. We show that murine carriage of this organism occurs in the bowel and nares, is acquired early in life, and can persist for months. Importantly, we observe the development of persistent and non-persistent gastrointestinal carriage states in genetically identical mice. We developed a needle- and antibiotic-free model in which we readily induced S. aureus colonisation of the gastrointestinal tract experimentally by environmental exposure. Using our experimental model, impact of adaptive immunity on S. aureus colonisation could be assessed. Vaccine efficacy to eliminate colonisation could also be investigated using this model.
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影响因子：
3.1
作者：
Gauguet, Stefanie;D'Ortona, Samantha;Pier, Gerald B.
通讯作者：
Pier, Gerald B.
影响因子：
9.4
作者：
Lebon, Ankie;Moll, Henriette A.;van Belkum, Alex
通讯作者：
van Belkum, Alex
影响因子：
9.4
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Gordon NC;Price JR;Cole K;Everitt R;Morgan M;Finney J;Kearns AM;Pichon B;Young B;Wilson DJ;Llewelyn MJ;Paul J;Peto TE;Crook DW;Walker AS;Golubchik T
通讯作者：
Golubchik T
影响因子：
158.5
作者：
Huang, Susan S.;Septimus, Edward;Platt, Richard
通讯作者：
Platt, Richard
影响因子：
7.3
作者：
Brown AF;Leech JM;Rogers TR;McLoughlin RM
通讯作者：
McLoughlin RM