Potential of Osteoblastic Cells Derived from Bone Marrow and Adipose Tissue Associated with a Polymer/Ceramic Composite to Repair Bone Tissue
Potential of Osteoblastic Cells Derived from Bone Marrow and Adipose Tissue Associated with a Polymer/Ceramic Composite to Repair Bone Tissue
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DOI：
10.1007/s00223-017-0282-3
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发表时间：
2017-09-01
影响因子：
4.2
通讯作者：
Rosa, Adalberto L.
中科院分区：
文献类型：
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作者：
Freitas, Gileade P.;Lopes, Helena B.;Rosa, Adalberto L.
促进骨再生的组织工程策略之一是细胞与生物材料的联合。在这种情况下，本研究的目的是评估细胞来源，无论是从骨髓或脂肪组织，影响与聚（偏乙烯-三氟乙烯）/钛酸钡（PVDF-TrFE/BT）膜相关的成骨细胞诱导的骨修复。从大鼠骨髓和脂肪组织中分离间充质干细胞（MSC），并通过检测几种表面标志物进行表征。此外，两种细胞群均在成骨条件下培养，并且观察到来自骨髓的MSC比来自脂肪组织的MSC更具成骨性。在植入PVDF-TrFE/BT膜并局部注射（1）从骨髓MSC分化的成骨细胞、（2）从脂肪组织MSC分化的成骨细胞或（3）磷酸盐缓冲盐水的大鼠颅骨缺损中评价骨修复。细胞注射后25天内，在骨缺损中检测到来自骨髓和脂肪组织的表达荧光素酶的成骨细胞，两种来源的细胞之间的荧光素信号无差异。证实了体外研究结果，骨髓中的成骨细胞与PVDF-TrFE/BT膜结合增加了骨形成，而脂肪组织中的成骨细胞并没有增强膜本身诱导的骨修复。基于这些发现，可以得出结论，通过在这种大鼠模型中将膜与细胞结合，细胞来源很重要，骨髓可能是更适合用于骨工程治疗的细胞来源。
One of the tissue engineering strategies to promote bone regeneration is the association of cells and biomaterials. In this context, the aim of this study was to evaluate if cell source, either from bone marrow or adipose tissue, affects bone repair induced by osteoblastic cells associated with a membrane of poly(vinylidene-trifluoroethylene)/barium titanate (PVDF-TrFE/BT). Mesenchymal stem cells (MSC) were isolated from rat bone marrow and adipose tissue and characterized by detection of several surface markers. Also, both cell populations were cultured under osteogenic conditions and it was observed that MSC from bone marrow were more osteogenic than MSC from adipose tissue. The bone repair was evaluated in rat calvarial defects implanted with PVDF-TrFE/BT membrane and locally injected with (1) osteoblastic cells differentiated from MSC from bone marrow, (2) osteoblastic cells differentiated from MSC from adipose tissue or (3) phosphate-buffered saline. Luciferase-expressing osteoblastic cells derived from bone marrow and adipose tissue were detected in bone defects after cell injection during 25 days without difference in luciferin signal between cells from both sources. Corroborating the in vitro findings, osteoblastic cells from bone marrow combined with the PVDF-TrFE/BT membrane increased the bone formation, whereas osteoblastic cells from adipose tissue did not enhance the bone repair induced by the membrane itself. Based on these findings, it is possible to conclude that, by combining a membrane with cells in this rat model, cell source matters and that bone marrow could be a more suitable source of cells for therapies to engineer bone.