Effect of post-anthesis waterlogging on biosynthesis and granule size distribution of starch in wheat grains
Effect of post-anthesis waterlogging on biosynthesis and granule size distribution of starch in wheat grains
复制标题
花后涝对小麦籽粒淀粉生物合成及粒径分布的影响
DOI：
10.1016/j.plaphy.2018.08.035
复制
发表时间：
2018
影响因子：
6.5
通讯作者：
Dong Jiang
中科院分区：
文献类型：
--
作者：
Qin Zhou;Mei Huang;Xin Huang;Jing Liu;Xiao Wang;Jian Cai;Tingbo Dai;Weixing Cao;Dong Jiang
涝渍是世界上许多多雨地区小麦生产中最常见的非生物胁迫类型之一。两种当地广泛种植的冬小麦（Triticum aestivumL.）以豫麦34号和扬麦9号为材料，采用盆栽试验研究了花后淹水对小麦淀粉合成及理化特性的影响。花后涝渍显著降低了籽粒的粒重，影响了淀粉组分的含量。涝渍处理下调了可溶性淀粉合成酶[SSS（EC 2.4.1.21）]基因的活性和表达，而上调了颗粒结合型淀粉合成酶I [GBSSI（EC：2.4.1.242）]基因的活性和表达。这进一步导致支链淀粉含量降低和直链淀粉含量增加。涝渍还导致成熟籽粒中淀粉粒数量减少，淀粉粒平均直径增大，这主要是由于A型淀粉粒体积频率百分比增加所致。淹水还降低了淀粉的峰值粘度和谷值粘度，但对崩解粘度和峰值时间没有影响。结果表明，花后涝渍条件下，小麦淀粉合成酶基因的表达改变导致了小麦淀粉颗粒大小分布的变化，进而影响了小麦淀粉的糊化特性。
Waterlogging is one of the most common abiotic stress types in wheat production in many rainy areas of the world. Two locally widely grown winter wheat (Triticum aestivumL. cv Yumai 34 and Yangmai 9) were subjected to post-anthesis waterlogging in a pot experiment to investigate the impacts of waterlogging on the starch synthesis and the physiochemical properties. Post-anthesis waterlogging significantly decreased grain weight and affected the content of starch components. Waterlogging down-regulated the activity and expression of genes encoding soluble starch synthase [SSS (EC 2.4.1.21)], while up-regulated those of the granule bound starch synthase I [GBSSI (EC:2.4.1.242)]. This further resulted in decreased amylopectin content and increased amylose content. Waterlogging also caused a reduction in the number of starch granules, while increased the mean diameter of starch granules in mature grains, which was mainly due to an increase in the volume frequency percent of the A-type starch granules. Waterlogging also lowered the peak viscosity and trough viscosity of starch, but did not affect the breakdown viscosity and peak time. We concluded that the modified expressions of the starch synthase encoding genes were responsible for the changed size distribution of starch granules, which finally affected the starch pasting properties of wheat growing under post-anthesis waterlogging conditions.