Distribution of microcystin-LR to testis of male Sprague–Dawley rats
Distribution of microcystin-LR to testis of male Sprague–Dawley rats
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DOI：
10.1007/s10646-013-1141-2
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发表时间：
2013-10
期刊：
影响因子：
2.7
通讯作者：
Lihui Wang;Xueting Wang;Zhirong Geng;Yuan Zhou;Yu Chen;Jiang Wu;Xiaodong Han
中科院分区：
文献类型：
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作者：
Lihui Wang;Xueting Wang;Zhirong Geng;Yuan Zhou;Yu Chen;Jiang Wu;Xiaodong Han
微囊藻毒素（MC）是一组由天然淡水蓝藻产生的环状七肽毒素。在 90 多种已鉴定的微囊藻毒素类似物中，微囊藻毒素-LR (MC-LR) 的含量最丰富，毒性也最大。我们之前的研究表明MC-LR表现出男性生殖毒性，但MC-LR在睾丸中的靶点仍不清楚。为此，本研究旨在阐明微囊藻毒素-LR是否可以分布到睾丸并探索睾丸中的靶细胞。在体内研究中，雄性 Sprague-Dawley 大鼠腹膜内注射 MC-LR，剂量为每天 300 μg/kg，持续 6 天。 MC-LR 在睾丸中检测到，主要在生精小管内，并通过蛋白质印迹进一步验证。通过LC-MS分析测定MC-LR的浓度，肝脏和睾丸中的MC-LR浓度分别为0.0252±0.0037和0.0056±0.0012μg/g干重。在体外研究中，将原代培养的精原细胞、支持细胞和间质细胞分别暴露于MC-LR，观察到MC-LR进入精原细胞和支持细胞，但不进入间质细胞。这些结果表明MC-LR的生殖毒性是由其在睾丸中的分布引起的。精原细胞和支持细胞是重要的靶细胞。
Microcystins (MCs) are a group of cyclic heptapeptide toxins produced by naturally freshwater cyanobacteria. Among more than 90 identified analogues of microcystins, microcystin-LR (MC-LR) is the most abundant and toxic. Our previous investigations indicated that MC-LR displays male reproductive toxicity, but the target of MC-LR in testes remains unclear. To this end, the present study is designed to elucidate whether microcystin-LR could be distributed to testes and explore the target cells in testes. In the in vivo study, male Sprague–Dawley rats were injected intraperitoneally with MC-LR at a dose of 300 μg/kg per day for 6 days. MC-LR was detected in testes, mainly within seminiferous tubules, which was further validated by Western blot. The concentrations of MC-LR were determined by LC–MS analysis, with a result of 0.0252 ± 0.0037 and 0.0056 ± 0.0012 μg/g dry weight in liver and testis respectively. In the in vitro study, Primary cultured spermatogonia, Sertoli cells and Leydig cells were exposed to MC-LR respectively, and MC-LR was observed to enter spermatogonia and Sertoli cells, but not Leydig cells. These results suggested that the reproductive toxicity of MC-LR were induced by its distribution in testis. Spermatogonia and Sertoli cells are important target cells.