Effect of heavy metal ion excess on sunflower leaves: Evidence for involvement of oxidative stress
Effect of heavy metal ion excess on sunflower leaves: Evidence for involvement of oxidative stress
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DOI：
10.1016/s0168-9452(96)04528-1
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发表时间：
1996-12-20
期刊：
影响因子：
5.2
通讯作者：
Tomaro, ML
中科院分区：
文献类型：
--
作者：
Gallego, SM;Benavides, MP;Tomaro, ML
研究了重金属离子毒害与植物细胞氧化胁迫的关系。将来自14天龄向日葵幼苗的叶段在含有0.5mM Fe（II）、Cu（II）或Cd（II）离子的溶液中在光照下孵育12小时。处理与金属离子研究产生的叶绿素和GSH含量下降，以及增加脂质过氧化和脂氧合酶活性。苯甲酸钠和甘露醇等自由基清除剂可防止叶绿素和GSH含量的下降以及脂质过氧化和脂氧合酶的增加。而Fe（II）和Cd（II）离子引起的超氧化物歧化酶活性下降，Cu（II）离子提高其水平。然而，所有三种金属离子引起的其他抗氧化酶（过氧化氢酶，抗坏血酸过氧化物酶，谷胱甘肽还原酶和脱氢抗坏血酸还原酶）的减少。自由基清除剂保护这些酶不被灭活，这些清除剂对超氧化物歧化酶活性没有影响。这些结果表明，过量的Fe（II），Cu（II）或Cd（II）离子在植物叶片中产生氧化损伤。版权所有（C）1996爱思唯尔科学爱尔兰有限公司
The relationship between heavy metal ion toxicity and oxidative stress in plant cells was studied. Leaf segments from 14 day old sunflower seedlings were incubated in solutions containing 0.5 mM Fe(II), Cu(II) or Cd(II) ions for 12 h in the light. Treatment with metal ions studied produced a decrease in chlorophyll and GSH contents as well as increases in lipid peroxidation and lipoxygenase activity. Free radical scavengers, such as sodium benzoate and mannitol, prevented the decrease in chlorophyll and GSH content and the lipid peroxidation and lipoxygenase increases. While Fe(II) and Cd(II) ions caused a decrease in superoxide dismutase activity, Cu(II) ions raised its level. However, all three metal ions caused decreases in other antioxidant enzymes (catalase, ascorbate peroxidase, glutathione reductase and dehydroascorbate reductase). Free radical scavengers protected these enzymes against inactivation, No effect of these scavengers was observed on superoxide dismutase activity, These results indicate that excess Fe(II), Cu(II) or Cd(II) ions produce oxidative damage in plant leaves. Copyright (C) 1996 Elsevier Science Ireland Ltd