Cytokeratin localization in toe pads of the anuran amphibian Philautus annandalii (Boulenger, 1906)
Cytokeratin localization in toe pads of the anuran amphibian Philautus annandalii (Boulenger, 1906)
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DOI：
10.1016/j.tice.2014.03.001
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发表时间：
2014-06-01
期刊：
影响因子：
2.6
通讯作者：
De, S. K.
中科院分区：
文献类型：
--
作者：
Chakraborti, S.;Nag, T. C.;De, S. K.
我们研究了细胞角蛋白分布和它们的性质在喜马拉雅树蛙Philautus annandalii趾垫。趾垫是指的扩展的尖端，并且显示出它们的腹侧表皮的粘附变化。组织成3-4排的趾垫表皮细胞具有角蛋白束，特别是在参与粘附的表面纳米结构中。免疫组织化学定位使用泛细胞角蛋白抗体显示，细胞角蛋白免疫反应性是最强的中基底细胞行的表皮，这平行于我们以前的超微结构观察致密的角蛋白束存在于这部分的表皮。表皮的其余部分（即，表面细胞层）显示很少的免疫反应性。免疫印迹分析表明，脚趾垫具有角蛋白显着的分子量为50 kDa。角蛋白5在趾垫表皮中的可能存在与其在哺乳动物表皮中的通常分布模式相关。(C)2014爱思唯尔有限公司版权所有。
We have examined cytokeratin distribution and their nature in toe pads of the Himalayan tree-frog Philautus annandalii. Toe pads are expanded tips of digits and show modifications of their ventral epidermis for adhesion. The toe pad epidermal cells, being organized into 3-4 rows, possess keratin bundles, especially in surface nanostructures that are involved in adhesion. Immunohistochemical localization using a pan-cytokeratin antibody revealed that cytokeratin immunoreactivity is the strongest in the mid- to basal cell rows of the epidermis, which parallels our previous ultrastructural observation of dense keratin bundles present in this part of the epidermis. The remainder of the epidermis (i.e., the superficial cell layer) showed little immunoreactivity. Immunoblot analysis revealed that toe-pads possessed keratins prominently in the molecular mass of 50 kDa. Possible presence of keratin 5 in toe pad epidermis has been correlated with its usual distribution pattern in mammalian epidermis. (C) 2014 Elsevier Ltd. All rights reserved.