ROLE OF THE PHYTOTOXIN CORONATINE IN THE INFECTION OF ARABIDOPSIS-THALIANA BY PSEUDOMONAS-SYRINGAE PV TOMATO
ROLE OF THE PHYTOTOXIN CORONATINE IN THE INFECTION OF ARABIDOPSIS-THALIANA BY PSEUDOMONAS-SYRINGAE PV TOMATO
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DOI：
10.1094/mpmi-8-0165
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发表时间：
1995-01-01
影响因子：
3.5
通讯作者：
DAVIS, KR
中科院分区：
文献类型：
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作者：
MITTAL, S;DAVIS, KR
植物毒素冠菌素在烟草致病假单胞菌致病力中的作用。通过比较症状发展、植物内细菌增殖和接种产生冠菌素的（Cor（+））P.s. pv、番茄DC 3000和冠菌素缺陷型（Cor（-））菌株DC 3661。Cor（+）菌株、P.s. PV.番茄DC 3000，经浸渗或浸渍接种后，发病症状严重，病株增殖4-6个对数级。番茄DC 3661没有产生任何病害症状，并且在浸渍的植物中仅增加1-1.5个对数，而在通过渗透接种的植物中，它引起轻微的症状，并且在4天的实验期内增加6个对数。使用天然宿主番茄进行的平行实验也得到了类似的结果。在用DC 3000或DC 3661浸润的拟南芥叶中由几种不同的防御相关基因编码的mRNA的积累的分析表明，Cor（-）菌株一致地诱导更高水平的这些转录物。这些结果表明，在更自然的接种条件下，需要冠菌素的产生来成功地用DC 3000感染拟南芥。冠菌素可能通过抑制防御相关基因的激活在感染的早期阶段发挥关键作用。
The role of the phytotoxin coronatine in the virulence of Pseudomonas syringae pv. tomato in Arabidopsis thaliana was evaluated by comparing symptom development, in planta bacterial multiplication, and the induction of defense-related genes in Arabidopsis plants inoculated with the coronatine-producing (Cor(+)) P. s. pv, tomato strain DC3000 and the coronatine-defective (Cor(-)) strain DC3661 by either infultration or dipping methods. The Cor(+) strain, P. s. pv. tomato DC3000, caused severe disease symptoms and multiplied by 4-6 logs after inoculation by either infiltration or dipping, P. s, pv. tomato DC3661 failed to produce any disease symptoms and multiplied by only 1-1.5 logs in dipped plants, whereas it caused mild symptoms and multiplied 6 logs over the 4-day experimental period in plants inoculated by infiltration. Parallel experiments using a natural host, tomato, yielded similar results. Analysis of the accumulation of mRNAs encoded by several distinct defense-related genes in Arabidopsis leaves infiltrated with either DC3000 or DC3661 demonstrated that the Cor(-) strain consistently induced higher levels of these transcripts, These results demonstrate that coronatine production is required under more natural inoculation conditions for the successful infection of Arabidopsis by DC3000, and that coronatine may play a critical role during the early stages of infection by suppressing the activation of defense-related genes.