DISTRIBUTION OF CGRP-IMMUNOREACTIVE, VIP-IMMUNOREACTIVE, D-BETA-H-IMMUNOREACTIVE, SP-IMMUNOREACTIVE, AND NPY-IMMUNOREACTIVE NERVES IN THE PERIOSTEUM OF THE RAT
DISTRIBUTION OF CGRP-IMMUNOREACTIVE, VIP-IMMUNOREACTIVE, D-BETA-H-IMMUNOREACTIVE, SP-IMMUNOREACTIVE, AND NPY-IMMUNOREACTIVE NERVES IN THE PERIOSTEUM OF THE RAT
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DOI：
10.1007/bf00319037
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发表时间：
1991-06-01
影响因子：
3.6
通讯作者：
ELDE, R
中科院分区：
文献类型：
--
作者：
HILL, EL;ELDE, R
鉴于周围神经在骨代谢中可能发挥的作用，在大鼠的膜骨(颅骨、下颌骨)和长骨(胫骨)的骨膜上，观察了降钙素基因相关肽(CGRP)、血管活性肠肽(VIP)、P物质(SP)、神经肽Y(NPY)和多巴胺-β-羟基酶(D-β-H)免疫反应神经纤维的形态。选择性切除交感神经或细口径初级传入神经的动物的骨膜也被检查以确定神经纤维的来源。我们发现骨膜有一致且通常致密的神经支配。颅骨和下颌骨的神经支配模式相似，神经网络分布于骨表面。胫骨骨膜内的神经沿纵轴方向排列，骨膜上的神经在骨膜上较多，在干骨中段较多。CGRP免疫反应纤维分布广泛、密集。不同口径和血管周围排列的CGRP免疫反应纤维群的存在表明，骨组织中的此类神经可能具有不同的功能。SP免疫反应阳性细胞位于骨膜浅层的细小的静脉曲张纤维网络中。与对照组相比，辣椒素处理组动物骨膜中CGRP和SP免疫反应神经纤维显著减少，提示这些神经的感觉来源。VIP免疫反应神经纤维分布于下颌骨和颅骨骨膜内，呈细小网状分布，个别呈细小曲张状分布。在胫骨骨膜中，可见较大的纤维网络。骨膜中的VIP免疫反应神经纤维与最靠近骨的细胞层中的血管和非血管成分相联系，提示VIP在骨膜组织中可能具有多种功能。NPY免疫反应纤维主要局限于血管成分，在骨衬细胞中偶见纤维。D-β-H免疫反应仅与血管相关。胍乙啶处理组动物骨膜中VIP、NPY和D-β-H免疫反应活性显著降低，提示这些神经来源于交感神经。
In light of the possible role peripheral nerves may play in bone metabolism, the morphology of calcitonin gene-related peptide (CGRP)-, vasoactive intestinal peptide (VIP)-, substance P (SP)-, neuropeptide Y (NPY)-, and dopamine-beta-hydroxylase (D-beta-H)-immunoreactive nerve fibers was examined in whole-mount preparations of periosteum of membranous bones (calvaria, mandible) and long bones (tibia) from the rat. Periosteum from animals treated to remove selectively either the sympathetic or fine-caliber primary afferent nerves was also examined to determine the origin of the nerve fibers. We found a consistent and often dense innervation of the periosteum. The innervation patterns of the calvaria and mandible were similar, with networks of nerves spread across the surface of the bone. Nerves in the tibial periosteum were oriented in the longitudinal axis and were more numerous at the epiphyses than in the mid-shaft region. CGRP-immunoreactive fibers were widely and densely distributed. The presence of populations of CGRP-immunoreactive fibers of differing calibers and perivascular arrangements suggests that such nerves in bone tissues may serve different functions. SP-immunoreactivity was present in a fine network of varicose fibers in the superficial layers of the periosteum. CGRP- and SP-immunoreactive nerve fibers were dramatically reduced in periosteum of capsaicin-treated animals as compared to controls, indicating the sensory origin of these nerves. VIP-immunoreactive nerve fibers were distributed in the periosteum of mandible and calvaria as small networks and individual fine varicose fibers. In tibial periosteum, larger networks of these fibers were visible. VIP-immunoreactive nerve fibers in the periosteum were associated with both vascular and nonvascular elements within the layers of cells closest to the bone, suggesting that VIP may serve more than one function in periosteal tissues. NPY-immunoreactive fibers were largely confined to vascular elements; occasional fibers were observed among the bone-lining cells. D-beta-H-immunoreactivity was associated only with blood vessels. VIP-, NPY-, and D-beta-H-immunoreactivities were dramatically reduced in the periosteum of guanethidine-treated animals, indicating the sympathetic origin of these nerves.