The Influence of the Environment on Cajal-Retzius Cell Migration
The Influence of the Environment on Cajal-Retzius Cell Migration
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DOI：
10.1093/cercor/bhp305
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发表时间：
2010-10-01
期刊：
影响因子：
3.7
通讯作者：
de Carlos, Juan A.
中科院分区：
文献类型：
--
作者：
Laura Ceci, Maria;Lopez-Mascaraque, Laura;de Carlos, Juan A.
在大脑皮层发育过程中，不同的细胞群通过前板切向迁移，从其起源部位向最终位置移动。 Cajal-Retzius（C-R）细胞是最早形成的细胞群之一，主要产生于不同的皮质下缘（CH）区域，它们沿着既定且平行的路线迁移以覆盖整个皮质幔。在这项研究中，我们提供的证据表明，-Retzius 细胞的表型及其一些迁移特征是在细胞生成的区域中指定的。然而，当异位植入时，这些细胞可以遵循新的迁移路线，这表明沿迁移路径本地提供的遗传线索非自主地影响来自 CH 不同部分的这些细胞的位置。通过在活的完整胚胎中进行表达荧光示踪剂的组织的 CH 植入见证了这一点。同样，由于 Pax6 的缺乏会影响迁徙环境中的一些指导因素，因此向小眼突变小鼠的下摆注射示踪剂特别能提供丰富的信息。因此，在这些动物中，C-R 细胞群杂乱无章，并且形成晚了 1 天，显示出基因表达的某些差异，这可能有助于解释这些破坏。
During cerebral cortex development, different cell populations migrate tangentially through the preplate, traveling from their site of origin toward their final positions. One of the earliest populations formed, the Cajal-Retzius (C-R) cells, is mainly generated in different cortical hem (CH) domains, and they migrate along established and parallel routes to cover the whole cortical mantle. In this study, we present evidence that the phenotype of -Retzius cells, as well as some of their migratory characteristics, is specified in the area where the cells are generated. Nevertheless, when implanted ectopically, these cells can follow new migratory routes, indicating that locally provided genetic cues along the migratory path nonautonomously influence the position of these cells emanating from different portions of the CH. This was witnessed by performing CH implants of tissue expressing fluorescent tracers in live whole embryos. In the same way, tracer injections into the hem of Small eye mutant mice were particularly informative since the lack of Pax6 affects some guidance factors in the migratory environment. As a result, in these animals, the C-R cell population is disorganized, and it forms 1 day late, showing certain differences in gene expression that might help explain these disruptions.