Isolation of a novel SUMO protein from tomato that suppresses EIX-induced cell death
Isolation of a novel SUMO protein from tomato that suppresses EIX-induced cell death
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DOI：
10.1046/j.1365-313x.1999.00547.x
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发表时间：
1999-09-01
期刊：
影响因子：
7.2
通讯作者：
Avni, A
中科院分区：
文献类型：
--
作者：
Hanania, U;Furman-Matarasso, N;Avni, A
用绿色木霉的乙烯诱导木聚糖酶（EIX）处理番茄或烟草品种，可迅速诱导植物防御反应，导致细胞程序性死亡。利用酵母双杂交系统，我们分离到一个新的蛋白，番茄小泛素相关修饰蛋白（T-SUMO），它特异性地与EIX相互作用。T-SUMO是一种细胞质蛋白，是泛素样蛋白家族的成员。它与人蛋白sentrin/SUMO 1同源，后者抑制肿瘤坏死因子诱导的细胞死亡。在有义方向表达T-SUMO的转基因植物抑制EIX诱导的乙烯生物合成和细胞死亡，而在反义方向，它们增强EIX诱导的乙烯生物合成。这些结果表明，T-SUMO参与介导EIX产生的信号，导致诱导植物防御反应。
Challenging tomato or tobacco varieties with ethylene-inducing xylanase (EIX) from the fungus Trichoderma viride causes rapid induction of plant defence responses leading to programmed cell death. Using the yeast two-hybrid system, we isolated a novel protein, tomato small ubiquitin-related modifier protein (T-SUMO), which specifically interacts with EIX. T-SUMO, a cytoplasmic protein, is a member of the ubiquitin-like protein family. It shows homology to human protein sentrin/SUMO1, which suppresses tumour necrosis factor-induced cell death. Transgenic plants that express T-SUMO in the sense orientation suppress EIX induction of ethylene biosynthesis and cell death, while in the antisense orientation they enhance EIX-induced ethylene biosynthesis. These results indicate that T-SUMO is involved in mediating the signal generated by EIX that leads to induction of plant defence responses.