Human glioma-mesenchymal extracellular matrix antigen defined by monoclonal antibody.
Human glioma-mesenchymal extracellular matrix antigen defined by monoclonal antibody.
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DOI：
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发表时间：
1983-06
期刊：
影响因子：
11.2
通讯作者：
M. Bourdon;C. Wikstrand;H. Furthmayr;T. Matthews;D. Bigner
中科院分区：
文献类型：
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作者：
M. Bourdon;C. Wikstrand;H. Furthmayr;T. Matthews;D. Bigner
已使用人培养细胞系和组织检查了由单克隆抗体81 C6定义的神经胶质瘤相关抗原的分布和定位。单克隆抗体81 C6是从用胶质细胞酸性蛋白阳性的人胶质瘤细胞系U-251 MG免疫的小鼠脾细胞的杂交瘤融合物中选择的。细胞表面放射免疫分析和吸收分析的结果表明，81 C6定义的胶质瘤间充质细胞外基质（GMEM）抗原表达的14 16胶质瘤，1 3神经母细胞瘤，1 7黑色素瘤，2 6肉瘤细胞系，和8 9培养的成纤维细胞系。GMEM不表达癌或骨髓淋巴细胞系检查。在中枢神经系统内，GMEM在11例胶质母细胞瘤中的10例中表达，但在6例星形细胞瘤中的5例以及正常成人和胎儿脑中通过过氧化物酶-抗过氧化物酶免疫组织学检测不到。在胶质母细胞瘤中，GMEM抗原定位于独特的肾小球样内皮增生和增生血管的基底膜。GMEM抗原也在3/3的胶质母细胞瘤细胞系和6/8的胶质母细胞瘤活检异种移植物中表达。在非中枢神经系统组织和肿瘤中，GMEM通过过氧化物酶-抗过氧化物酶免疫组织学在正常肝血窦、脾红髓血窦、肾髓质小管和肾小球系膜中发现，并且与几种未分化肿瘤的血管和基质成分相关。GMEM抗原不同于先前描述的纤连蛋白、层粘连蛋白、I至V型胶原、透明质酸、硫酸软骨素和肝素的形式，如通过吸收分析和组织中的免疫组织学定位所确定的。GMEM在胶质母细胞瘤中表达，但在正常脑中不表达，与胶质母细胞瘤增殖内皮基底膜相关，以及在胶质母细胞瘤细胞系和裸鼠移植瘤中表达，表明GMEM可能是体内和体外胶质瘤的有用标记物。
The distribution and localization of a glioma-associated antigen defined by monoclonal antibody 81C6 has been examined using human cultured cell lines and tissues. Monoclonal antibody 81C6 was selected from a hybridoma fusion of spleen cells of mice immunized with the glial fibrillary acidic protein-positive human glioma cell line U-251 MG. Results of cell surface radioimmunoassay and absorption analysis demonstrated that 81C6 defined a glioma-mesenchymal extracellular matrix (GMEM) antigen expressed by 14 of 16 gliomas, 1 of 3 neuroblastomas, 1 of 7 melanomas, 2 of 6 sarcoma cell lines, and 8 of 9 cultured fibroblast lines. GMEM was not expressed by carcinoma or by the myeloid-lymphoid cell lines examined. Within the central nervous system, GMEM was expressed in 10 of 11 glioblastomas but was undetected in 5 of 6 astrocytomas and in normal adult and fetal brain by peroxidase-antiperoxidase immunohistology. In glioblastomas, the GMEM antigen was localized to basement membranes of the distinctive glomeruloid endothelial proliferations and hyperplastic blood vessels. The GMEM antigen was also expressed in 3 of 3 glioblastoma cell lines and 6 of 8 glioblastoma biopsy xenografts in athymic nude mice. Among non-central nervous system tissues and tumors, GMEM was found by peroxidase-antiperoxidase immunohistology in normal liver sinusoids, spleen red pulp sinusoids, kidney medullary tubule interstitium, and glomerular mesangium and in association with vascular and stromal elements of several undifferentiated tumors. The GMEM antigen is distinct from previously described forms of fibronectin, laminin, collagen types I to V, hyaluronic acid, chondroitin sulfate, and heparin, as determined by absorption analysis and immunohistological localization in tissues. The expression of GMEM in glioblastoma but not normal brain, association with glioblastoma-proliferative endothelium basement membranes, and expression in glioblastoma cell lines and nude mouse xenografts suggest that GMEM may be a useful marker of gliomas in vivo and in vitro.