Transcription from the RAG1 locus marks the earliest lymphocyte progenitors in bone marrow
Transcription from the RAG1 locus marks the earliest lymphocyte progenitors in bone marrow
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DOI：
10.1016/s1074-7613(02)00366-7
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发表时间：
2002-08-01
期刊：
影响因子：
32.4
通讯作者：
Kincade, PW
中科院分区：
文献类型：
--
作者：
Igarashi, H;Gregory, SC;Kincade, PW
可行的LIN（ - ）CD27（+）C-KIT（HI）SCA-1（HI）GFP（+）细胞从杂合RAG1/GFP敲击蛋白小鼠中恢复的细胞通过B，T和NK细胞谱系的B，T和NK细胞谱系的先前阶段进展。与GFP（ - ）队列相比，具有活性RAG1基因座的细胞中的髓样或红细胞潜力最少。与TDT（+）细胞的部分重叠表明，不同步获得独特的早期淋巴细胞特征。在建立典型的基因表达淋巴样谱系模式之前，Ig基因的重排开始，并在大量的细胞中暂时发生RAG1基因座的激活，该细胞注定为NK细胞。这些早期的淋巴细胞祖细胞（ELP）与干细胞不同，先前描述的刺激细胞或与其他血细胞谱系相对应的祖细胞。
Viable Lin(-) CD27(+) c-kit(Hi) SCa-1(Hi) GFP(+) cells recovered from heterozygous RAG1/GFP knockin mice progressed through previously defined stages of B, T, and NK cell lineage differentiation. In contrast to the GFP(-) cohort, there was minimal myeloid or erythroid potential in cells with an active RAG1 locus. Partial overlap with TdT(+) cells suggested that distinctive early lymphocyte characteristics are not synchronously acquired. Rearrangement of Ig genes initiates before typical lymphoid lineage patterns of gene expression are established, and activation of the RAG1 locus transiently occurs in a large fraction of cells destined to become NK cells. These early lymphocyte progenitors (ELP) are distinct from stem cells, previously described prolymphocytes, or progenitors corresponding to other blood cell lineages.