Quantum dot-based lateral flow immunoassay for detection of chloramphenicol in milk
Quantum dot-based lateral flow immunoassay for detection of chloramphenicol in milk
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DOI：
10.1007/s00216-013-6876-3
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发表时间：
2013-05-01
影响因子：
4.3
通讯作者：
Dzantiev, Boris B.
中科院分区：
文献类型：
--
作者：
Berlina, Anna N.;Taranova, Nadezhda A.;Dzantiev, Boris B.
建立了一种新型快速（20 min）荧光侧流法检测牛奶中氯霉素（CAP）的方法。选择的形式是结合抑制试验。采用发射峰为625 nm的水溶性量子点作为标记。牛奶样品用磷酸盐缓冲液稀释20%以消除基质效应。测定结果可在紫外光激发下用肉眼观察或用便携式功率依赖光度计记录。第二种方法的CAP检测限为0.2 ng/mL，定量限为0.3 ng/mL。
A novel rapid (20 min) fluorescent lateral flow test for chloramphenicol (CAP) detection in milk was developed. The chosen format is a binding-inhibition assay. Water-soluble quantum dots with an emission peak at 625 nm were applied as a label. Milk samples were diluted by 20 % with phosphate buffer to eliminate the matrix effect. The result of the assay could be seen by eye under UV light excitation or registered by a portable power-dependent photometer. The limit of CAP detection by the second approach is 0.2 ng/mL, and the limit of quantitation is 0.3 ng/mL.