Can a leukocyte depletion filter (LDF) reduce the risk of reintroduction of hepatocellular carcinoma cells?
Can a leukocyte depletion filter (LDF) reduce the risk of reintroduction of hepatocellular carcinoma cells?
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DOI：
10.1002/lt.20346
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发表时间：
2005-03-01
影响因子：
4.6
通讯作者：
Lee, SK
中科院分区：
文献类型：
--
作者：
Gwak, MS;Lee, KW;Lee, SK
在肝细胞癌（HCC）患者的肝移植过程中，当使用自体输血装置时，理论上可以将HCC引入体循环。一些报告表明，某些类型的白细胞描绘过滤器（LDFs）可以完全降低重新引入某些类型的肿瘤细胞的风险。在这项研究中，我们通过使用非常灵敏的检测方法测试了LDF（RCEZ 1 T，Pall Biomedical Co，NY，USA）在体外降低再引入HCC细胞的风险的能力。我们将试验组分为6组：组I为10个细胞，组II为20个细胞，组III为2 × 10（3）个细胞，组IV为2 × 10（5）个细胞，组V为2 × 10（6）个细胞，组VI为2 × 10（7）个细胞。使用重力使200 mL盐水中的计数细胞通过RCEZ 1 T。为了鉴定细胞的存在，将沉淀物重悬，并进行聚合酶链反应（PCR）。使用管家基因甘油醛-3-磷酸脱氢酶（GAPDH）作为引物。在组I和组II中，HCC细胞在所有实验中被完全过滤。然而，在组III、IV和V中，在少数重复实验中HCC细胞未被完全过滤，肿瘤细胞的未过滤率在8%和20%之间。在第VI组中，在所有重复实验中HCC细胞未被完全过滤。总之，RCEZ 1 T滤器显著降低了HCC细胞再引入的风险。然而，在高HCC细胞负荷下，过滤器不能完全去除所有肿瘤细胞。需要进一步的研究来评估临床环境中的影响。
During liver transplantation for hepatocellular carcinoma (HCC) patients, HCC could theoretically be introduced into the systemic circulation when salvaged blood is used with an autotransfusion device. Several reports have shown that some types of leukocyte depiction filters (LDFs) could completely reduce the risk for reintroducing some types of tumor cells. In this study, we tested the ability of the LDF (RCEZ1T, Pall Biomedical Co, NY, USA) to reduce the risk for reintroducing HCC cells in vitro by using a very sensitive detection method. We divided the test group into 6 groups: group I was 10 cells, group II was 20 cells, group III was 2 X 10(3) cells, group IV was 2 X 10(5) cells, group V was 2 X 10(6) cells, and group VI was 2 X 10(7) cells. The counted cells in 200 mL saline were passed through the RCEZ1T using the force of gravity. To identify the presence of cells, the pellet was resuspended, and polymerase chain reaction (PCR) was performed. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a housekeeping gene, was used as a primer. In groups I and II, the HCC cells were completely filtered in all experiments. However, in groups III, IV, and V, the HCC cells were not completely filtered in a few of the repeated experiments, with the unfiltered rate of tumor cells being between 8% and 20%. In group VI, the HCC cells were not completely filtered in all the repeated experiments. In conclusion, the RCEZ1T filter markedly reduced the risk for reintroduction of HCC cells. However, at high HCC cell load the filter cannot completely remove all the tumor cells. Further studies are required to assess the impact in clinical settings.