Evaluation of cytokines in type 1 diabetes patients with and without retinopathy
Evaluation of cytokines in type 1 diabetes patients with and without retinopathy
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DOI：
10.5935/1676-2444.20170001
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发表时间：
2017-02-01
影响因子：
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通讯作者：
Domingueti, Caroline P.
中科院分区：
文献类型：
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作者：
Cardoso, Jéssica F.;Gomes, Karina B.;Domingueti, Caroline P.
摘要引言：糖尿病是世界上最大的公共卫生问题之一;这种疾病的众多并发症是非常令人关注的焦点，因为它们与高发病率和死亡率有关。在糖尿病的并发症中，糖尿病视网膜病变是最常见的微血管并发症。 目的：评估炎症细胞因子白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）以及抗炎细胞因子白细胞介素-10（IL-10）的血浆水平与糖尿病视网膜病变的相关性以及这些细胞因子的血浆水平的相关性。 材料和方法：125例1型糖尿病患者纳入本研究，其中55例有视网膜病变，70例无视网膜病变。流式细胞术检测细胞因子IL-6、TNF-α、IFN-γ和IL-10。 结果和讨论：未观察到细胞因子IL-6、TNF-α、IFN-γ和IL-10水平与视网膜病变的显著相关性，这可能是因为对血浆样本进行了测量，而不是对玻璃体液进行测量。而细胞因子之间呈正相关（p < 0.001）。 结论：细胞因子IL-6、TNF-α、IFN-γ和IL-10之间呈正相关，提示它们可能共同参与糖尿病血管并发症的发生和发展。
ABSTRACT Introduction: Diabetes mellitus is one of the greatest public health problems in the world; the numerous complications of this disease are the focus of great concern, as they are related to high morbidity and mortality. Among the complications of diabetes, diabetic retinopathy is the most common microvascular complication. Objectives: Evaluate the association of plasma levels of inflammatory cytokines interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ), and anti-inflammatory cytokine interleukin-10 (IL-10) with diabetic retinopathy and the correlation of plasma levels of these cytokines together. Material and method: 125 patients with type 1 diabetes mellitus were included in this study - 55 with retinopathy and 70 without retinopathy. Cytokines IL-6, TNF-α, IFN-γ and IL-10 were determined by flow cytometry. Results and discussion: A significant association of the levels of cytokines IL-6, TNF-α, IFN-γ and IL-10 with retinopathy was not observed, possibly because measurements were performed on plasma samples, but not on vitreous humor. However, a positive correlation of cytokines among themselves (p < 0.001) was observed. Conclusion: Cytokines IL-6, TNF-α, IFN-γ and IL-10 presented a positive correlation among themselves, what suggests that they may act together in the development and progression of vascular complications in diabetes patients.