Metabolism of cyanide by Phanerochaete chrysosporium.
Metabolism of cyanide by Phanerochaete chrysosporium.
复制标题
黄孢原毛平革菌对氰化物的代谢。
DOI：
10.1016/0003-9861(91)90604-h
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发表时间：
1991
影响因子：
3.9
通讯作者：
Aust,SD
中科院分区：
文献类型：
--
作者：
Shah,MM;Grover,TA;Aust,SD
氰化钠对白腐菌黄孢原毛平革菌木素过氧化物酶H2氧化藜芦醇(3，4-二甲氧基苯甲醇)的反应有很强的抑制作用。其I50约为2-3μm，而氰化钠则与天然酶结合，其解离常数Kd约为10μm，氰化物对LIP H_2的藜芦醇氧化酶活性的抑制是可逆的。木质素分解培养。黄孢子菌对氰化物的矿化速度与氰化物的浓度成正比，达到2 mm。LiP H_2与H_2O_2和氰化钠反应生成叔丁基-α-苯基硝酮-氰基自由基加合物。用~(13)C标记氰化物证实了自旋加合物的真实性。6天大的真菌培养物对氰化钠毒性的耐受性比孢子强。毒性测量是基于氰化钠对真菌呼吸的影响，通过[14C]葡萄糖到[14C]CO2的代谢来确定。我们认为这种成熟真菌的耐受性是由于它对氰化物的矿化能力，这种真菌可能在处理氰化物污染的环境污染场地方面有效。
The oxidation of veratryl alcohol (3,4-dimethoxybenzyl alcohol) by lignin peroxidase H2 (LiP H2) from the white rot fungusPhanerochaete chrysosporiumwas strongly inhibited by sodium cyanide. TheI50was estimated to be about 2–3 μm. In contrast, sodium cyanide binds to the native enzyme with an apparent sodium cyanide dissociation constantKdof about 10 μm. Inhibition of the veratryl alcohol oxidase activity of LiP H2 by cyanide was reversible. Ligninolytic cultures ofP. chrysosporiummineralized cyanide at a rate that was proportional to the concentration of cyanide to 2 mm. TheN-tert-butyl-α-phenylnitrone—cyanyl radical adduct was observed by ESR spin trapping upon incubation of LiP H2 with H2O2and sodium cyanide. The identity of the spin adduct was confirmed using13C-labeled cyanide. Six-day-old cultures of the fungus were more tolerant to sodium cyanide toxicity than spores. Toxicity measurements were based on the effect of sodium cyanide on respiration of the fungus as determined by the metabolism of [14C]glucose to [14C]CO2. We propose that this tolerance of the mature fungus was due to its ability to mineralize cyanide and that this fungus might be effective in treating environmental pollution sites contaminated with cyanide.