Low-Intensity Ultrasound Stimulation in Distraction Osteogenesis in Rabbits
Low-Intensity Ultrasound Stimulation in Distraction Osteogenesis in Rabbits
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DOI：
10.1097/01.blo.0000093043.56370.5a
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发表时间：
2003-12
影响因子：
4.2
通讯作者：
M. Uglow;Rachel A. Peat;M. Hile;L. Bilston;E. J. Smith;D. Little
中科院分区：
文献类型：
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作者：
M. Uglow;Rachel A. Peat;M. Hile;L. Bilston;E. J. Smith;D. Little
低强度脉冲超声已被证明可以加速骨折愈合。这个实验研究了它在分散注意力中的可能作用。34只新西兰白色兔进行牵张成骨，随后进行低强度脉冲超声治疗。十七只动物关闭超声传感器（对照组）。术后4周和6周，使用定量计算机断层扫描和四点机械测试分析了胫骨。在4周时，每组中的两个时间点进行组织学分析。在骨矿物质含量、横截面积和强度方面，超声处理组和对照组的再生骨之间没有显著差异。未观察到再生骨近端和远端骨质减少显著减少。组织学观察显示骨体积分数没有差异，但超声处理的再生骨似乎具有较少的厚度增加的骨小梁和较少的破骨细胞。超声波的调节作用可能是通过作用于软骨细胞加速软骨内成骨，而牵张成骨主要是膜内的。虽然超声波被证明是有效的，在不受约束的系统，如石膏，目前的研究不支持的作用，低强度脉冲超声波作为一个辅助的患者有牵引成骨在刚性固定器。需要更多的研究来明确支持在这种情况下使用低强度脉冲超声。
Low-intensity pulsed ultrasound has been shown to accelerate fracture healing. This experiment investigated its possible role in distraction. Thirty-four New Zealand White rabbits had distraction osteogenesis, followed by low-intensity pulsed ultrasound therapy. Seventeen animals had the ultrasound transducer switched off (controls). Four and 6 weeks postoperatively, tibiae were analyzed using quantitative computed tomography and four-point mechanical testing. Two tibiae from each group had histologic analysis at 4 weeks. No significant differences were identified between regenerates of ultrasound-treated and control groups with respect to bone mineral content, cross-sectional area, and strength. No significant reductions in osteopenia proximal and distal to the regenerate were observed. Histologic observation showed no differences in bone volume fraction, but ultrasound-treated regenerates appeared to have fewer trabeculae of increased thickness, and fewer osteoclasts. The modulation by ultrasound may occur by accelerating endochondral ossification through action on chondrocytes, yet distraction osteogenesis is largely intramembranous. Although ultrasound is proven to be effective in unconstrained systems such as plaster, the current study does not support the role of low-intensity pulsed ultrasound as an adjunct for patients having distraction osteogenesis in a rigid fixator. Additional research is needed to definitively support the use of low-intensity pulsed ultrasound in such situations.