Gene amplification in gastric and esophageal adenocarcinomas.
Gene amplification in gastric and esophageal adenocarcinomas.
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DOI：
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发表时间：
1990-10
期刊：
影响因子：
11.2
通讯作者：
Jane Houldsworth;Carlos Cordon-Cardo;Marc Ladanyi;David P. Kelsen;R. Chaganti
中科院分区：
文献类型：
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作者：
Jane Houldsworth;Carlos Cordon-Cardo;Marc Ladanyi;David P. Kelsen;R. Chaganti
在美国28例低分化至中度分化的胃/食管腺癌中，我们在21%的肿瘤中检测到基因扩增。利用改进的DNA凝胶内再生法检测7-8拷贝的扩增DNA序列，我们鉴定出3个具有扩增DNA序列的胃肿瘤，并通过Southern杂交分析证实HER-2/neu在这些标本中扩增大于5倍。p185 HER-2/neu抗体对肿瘤组织切片进行免疫组化染色显示，只有3例相应HER-2/neu基因扩增的胃腺癌表现出膜免疫反应性。在2个肿瘤中发现了c-met的扩增，这是首次在新鲜胃肿瘤组织中检测该原癌基因的多个拷贝。在一个肿瘤中观察到c-erbB扩增，而在这一系列腺癌中未发现int-2扩增的证据。
In a United States series of 28 gastric/esophageal adenocarcinomas of poorly to moderately differentiated histopathology, we detected gene amplification in 21% of the tumors. Using the modified DNA in-gel renaturation assay to detect down to 7-8 copies of amplified DNA sequences, we identified 3 gastric tumors with amplified DNA sequences and confirmed by Southern hybridization analysis that HER-2/neu was amplified greater than 5-fold in these specimens. Immunohistochemical staining of tumor tissue sections with p185 HER-2/neu antibodies demonstrated that only the 3 gastric adenocarcinomas with corresponding HER-2/neu gene amplification displayed membrane immunoreactivity. Amplification of c-met was identified in 2 tumors, and this was the first study to assay for multiple copies of this protooncogene in fresh gastric tumor tissues. Amplification of c-erbB was observed in one tumor, and no evidence for amplification of int-2 was obtained in this series of adenocarcinomas.