Long non-coding RNAs expressed in pancreatic ductal adenocarcinoma and lncRNA BC008363 an independent prognostic factor in PDAC
Long non-coding RNAs expressed in pancreatic ductal adenocarcinoma and lncRNA BC008363 an independent prognostic factor in PDAC
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DOI：
10.1016/j.pan.2014.07.013
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发表时间：
2014-09-01
期刊：
影响因子：
3.6
通讯作者：
Sun, Yongwei
中科院分区：
文献类型：
--
作者：
Li, Jiao;Liu, Dejun;Sun, Yongwei
工作背景：长链非编码RNA（longnon-codingRNA，IncRNA）是一类新型的mRNA样转录物，不具有蛋白质编码能力，但具有表观遗传学和基因调控等多种功能。方法：采用基因芯片技术检测胰腺导管腺癌（pancreatic ductal adenocarcinoma，PDAC）组织中lncRNA的表达，并采用实时荧光定量PCR技术检测30例胰腺导管腺癌患者手术切除标本中lncRNA的表达。结果：在PDAC患者中，有1881条IncRNA表达上调，3369条IncRNA表达下调（变化倍数>= 4.0或3.0），其中1881条表达上调，3369条表达下调（变化倍数>= 4.0或3.0），差异有统计学意义（P < 0.05）。
Background: Long non-coding RNAs (IncRNAs) are a novel class of mRNA-like transcripts with no protein coding capacity, but with a variety of functions including roles in epigenetics and gene regulation. In recent reports, the aberrant expression of lncRNAs has been associated with human cancers, suggesting a critical role in tumorigenesis.Method: In the present study, we analyzed mRNA and IncRNA expression in pancreatic ductal adenocarcinoma (PDAC) by microarray platform, and analyzed IncRNA expression from 30 patients who underwent pancreatectomy for pancreatic ductal adenocarcinoma by real-time PCR. We also investigated the relationship between the expression of BC008363 and the prognosis of PDAC patients by Kaplan-Meier analyses.Results: Based on the microarray data, 1881 IncRNAs were upregulated and 3369 IncRNAs were down-regulated (fold change >= 4.0 or