Bioprocessing of citrus waste peel for induced pectinase production by Aspergillus niger; its purification and characterization
Bioprocessing of citrus waste peel for induced pectinase production by Aspergillus niger; its purification and characterization
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DOI：
10.1016/j.jrras.2015.11.003
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发表时间：
2016-04-01
影响因子：
1.7
通讯作者：
Nowrouzi, Azin
中科院分区：
文献类型：
--
作者：
Ahmed, Ishtiaq;Zia, Muhammad Anjum;Nowrouzi, Azin
农业工业残留物主要由复杂的多糖组成，这些多糖可以加强微生物的生长，以生产工业上重要的酶。果胶酶是细菌、真菌和植物中分布最广的酶之一。以橙子皮渣为碳源的Czapeck培养基进行深层发酵，尼日尔曲霉产酶能力最强。在部分优化培养中，在发酵第5天后，在30 ℃下，在pH 5.5和底物浓度（4%）的橙子废皮培养基中，显示出最大的酶产率（117.1 +/-3.4 μ M/mL/min）。用硫酸铵沉淀和离子交换层析对酶进行纯化。经凝胶过滤层析纯化后，纯化倍数为5.59，比活为97.2U/mg，回收率为12.96%。纯化后的果胶酶分子量为1000 - 10000。SDS-PAGE分析表明，尼日尔变蛋白分子量为30 kDa。酶活测定结果表明，纯化的果胶酶在pH = 7和55 ℃时活性最高。果胶酶在低浓度的廉价底物存在下的最大产量使得该酶可用于工业部门，特别是纺织和果汁工业。Copyright（C）2015，The Egyptian Society of Radiation Sciences and Applications.制作和托管由Elsevier B. V.这是一个开放获取的文章在CC BY-NC-ND许可证（http：//creativecommons.org/licenses/by-nc-nd/4.0/）。
Agro-industrial residues are primarily composed of complex polysaccharides that strengthen microbial growth for the production of industrially important enzymes. Pectinases are one of the most widely disseminated enzymes in bacteria, fungi and plants. Czapeck media supplemented with orange waste peel as carbon source under submerged fermentation process Aspergillus niger presenting the preeminent enzymatic production. On partial optimization culture showed the maximum enzyme yield (117.1 +/- 3.4 mu M/mL/min) at 30 degrees C in an orange waste peel medium having pH 5.5 and substrate concentration (4%) after 5th day of fermentation. The produced enzyme was purified by ammonium sulfate precipitation and ion exchange chromatography. A purification fold of 5.59 with specific activity and % recovery of 97.2 U/mg and 12.96% was achieved respectively after gel filtration chromatographic technique. The molecular weight of purified pectinase from A. niger was 30 kDa evidenced by SDS-PAGE. Pectinase activity profile showed purified enzyme was optimally active at pH = 7 and 55 degrees C. The maximum production of pectinase in the presence of cheaper substrate at low concentration makes the enzyme useful in industrial sectors especially for textile and juice industry. Copyright (C) 2015, The Egyptian Society of Radiation Sciences and Applications. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).