Experimental and Computational Considerations in the Study of RNA-Binding Protein-RNA Interactions.
Experimental and Computational Considerations in the Study of RNA-Binding Protein-RNA Interactions.
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RNA 结合蛋白-RNA 相互作用研究中的实验和计算注意事项。
DOI：
10.1007/978-3-319-29073-7_1
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发表时间：
2016
影响因子：
--
通讯作者：
Yeo,GeneW
中科院分区：
文献类型：
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作者：
VanNostrand,EricL;Huelga,StephanieC;Yeo,GeneW
RNA转录后，它经历了各种加工步骤，可以改变编码的蛋白质序列（通过选择性剪接和RNA编辑），调节RNA的稳定性，并控制亚细胞定位，时间和翻译速率。最近基因组学技术的爆炸性发展使得能够以无偏见的方式对RNA加工进行转录组范围的分析。然而，它也带来了在开发改进的方法来探测不同的处理步骤方面的实验挑战，以及在数据存储，处理和分析工具方面的计算挑战，以实现基因组学时代的大规模解释。在这一章中，我们将回顾RNA加工各个方面的实验技术和挑战，以及最近的努力，开发分析整合多个数据源和技术来推断RNA调控网络。
After an RNA is transcribed, it undergoes a variety of processing steps that can change the encoded protein sequence (through alternative splicing and RNA editing), regulate the stability of the RNA, and control subcellular localization, timing, and rate of translation. The recent explosion in genomics techniques has enabled transcriptome-wide profiling of RNA processing in an unbiased manner. However, it has also brought with it both experimental challenges in developing improved methods to probe distinct processing steps, as well as computational challenges in data storage, processing, and analysis tools to enable large-scale interpretation in the genomics era. In this chapter we review experimental techniques and challenges in profiling various aspects of RNA processing, as well as recent efforts to develop analyses integrating multiple data sources and techniques to infer RNA regulatory networks.