LARYNGEAL SYNKINESIS FOLLOWING REINNERVATION IN THE RAT - NEUROANATOMICAL AND PHYSIOLOGICAL STUDY USING RETROGRADE FLUORESCENT TRACERS AND ELECTROMYOGRAPHY
LARYNGEAL SYNKINESIS FOLLOWING REINNERVATION IN THE RAT - NEUROANATOMICAL AND PHYSIOLOGICAL STUDY USING RETROGRADE FLUORESCENT TRACERS AND ELECTROMYOGRAPHY
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DOI：
10.1177/000348949110001003
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发表时间：
1991-10-01
影响因子：
1.4
通讯作者：
COLTRERA, MD
中科院分区：
文献类型：
--
作者：
FLINT, PW;DOWNS, DH;COLTRERA, MD
对成年雄性大鼠喉返神经切除和再神经移植前后喉部运动神经元的功能组织进行了观察。采用荧光探针逆行双标记技术，利用Bioquant三维成像系统获得标记神经元的数量和位置。肌电图显示神经再生。在9只对照动物中，载体分析显示环杓后（PCA）肌运动神经元与环杓甲状腺和环杓外侧（TA/LCA）肌运动神经元分离显著（p < 0.05）。PCA运动神经元位于NA的腹内侧，TA/LCA运动神经元位于NA的背侧。喙尾分离不显著。肌电图显示PCA的相电活动与呼吸同步，TA/LCA的相电活动与吞咽同步。在喉返神经切除和原发性神经缝合后存活15周的4只动物中，NA内的功能组织丢失，TA/LCA肌肉以及PCA中可见与呼吸同步的相动单元活动。载体分析显示，PCA和TA/LCA的再神经运动神经元位于背外侧，与对照组TA/LCA的运动神经元相似。这些结果表明，在神经移植后，NA内喉运动神经元的地形组织发生了变化，在神经缝合部位出现了随机组织。
The functional organization of laryngeal motoneurons in the nucleus ambiguus (NA) was evaluated in adult male rats before and after recurrent laryngeal nerve section and reinnervation. Using retrograde double labeling techniques with fluorescent probes, we obtained the number and position of labeled neurons by using the Bioquant 3-D imaging system. Reinnervation was documented by electromyography. In nine control animals vector analysis revealed significant (p < .05) separation of the posterior cricoarytenoid (PCA) muscle motoneurons and the thyroarytenoid and lateral cricoarytenoid (TA/LCA) muscle motoneurons. The PCA motoneurons were positioned ventromedially in the NA, and TA/LCA motoneurons were found dorsolaterally in the NA. Rostral-caudal separation was not significant. Electromyography revealed phasic electrical activity synchronous with respiration in the PCA, and activity synchronous with deglutition in the TA/LCA. In four animals surviving 15 weeks following recurrent laryngeal nerve section and primary neurorrhaphy, functional organization within the NA was lost and phasic motor unit activity synchronous with respiration was seen in the TA/LCA muscle as well as the PCA. Vector analysis revealed the reinnervating motoneurons for both the PCA and TA/LCA to be positioned dorsolaterally, similar to the control group TA/LCA motoneurons. These findings demonstrate a shift in the topographic organization of laryngeal motoneurons within the NA following reinnervation, with random organization occurring at the neurorrhaphy site.