INFANT RAT MODEL OF ATTENUATION FOR RECOMBINANT INFLUENZA-VIRUSES PREPARED FROM COLD-ADAPTED ATTENUATED A/ANN-ARBOR/6/60
INFANT RAT MODEL OF ATTENUATION FOR RECOMBINANT INFLUENZA-VIRUSES PREPARED FROM COLD-ADAPTED ATTENUATED A/ANN-ARBOR/6/60
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DOI：
10.1128/iai.38.2.610-619.1982
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发表时间：
1982-01-01
影响因子：
3.1
通讯作者：
POTTER, CW
中科院分区：
文献类型：
--
作者：
ALI, M;MAASSAB, HF;POTTER, CW
测定了6株野生型甲型流感病毒和21株由野生型病毒和冷适应A/安阿伯/6/60病毒制备的重组株对幼鼠的致病性。因此，在鼻内感染后5天连续测量病毒感染动物的鼻甲和肺中存在的病毒滴度，并在病毒感染后48小时测量病毒株促进流感嗜血杆菌随后全身细菌感染的能力。参考病毒株的遗传组成和对志愿者的病毒毒力，对获得的结果进行了评估。毒力强的病毒在大鼠鼻甲中生长到相对高的滴度，并显著促进H.流感。减毒菌株生长至较低滴度且不能促进系统性H。流感感染。对于测试的菌株，结果显示减毒和强毒株的明显差异，并且该模型是对人类毒力的可靠指示。虽然强毒株在大鼠肺中的滴度往往比减毒株高，但也有例外，这种测量方法不能可靠地区分强毒株和减毒株。显然，幼鼠可用于评估冷适应重组流感病毒株的毒力，因此，它们可促进此类疫苗生产株的开发。
The pathogenicity of 6 wild-type influenza A viruses and 21 recombination strains prepared from wild-type viruses and cold-adapted A/Ann Arbor/6/60 virus for infant rats was determined. Thus, the titers of virus present in the turbinates and lungs of virus-infected animals was measured serially for 5 days after intranasal infection, and the ability of virus strains to promote subsequent systemic bacterial infection by Haemophilus influenzae was measured at 48 h after virus infection. The results obtained were assessed with reference to the genetic constitution of the virus strains and to virus virulence for volunteers. Virulent viruses grew to relatively high titers in rat turbinates and significantly promoted systemic infection by H. influenzae. Attenuated strains grew to lower titers and failed to promote systemic H. influenzae infection. For the strains tested, the results showed clear differences for attenuated and virulent strains, and the model was a reliable indication of virulence for humans. Although the virulent strains tended to grow to higher titers in rat lungs than did attenuated strains, exceptions were found, and this measurement could not reliably discriminate virulent and attenuated virus strains. Evidently, infant rats can be used to assess the virulence of cold-adapted recombinant influenza virus strains, and thus, they can facilitate the development of such strains for vaccine production.