The determination of mercury in whole blood and urine by inductively coupled plasma mass spectrometry
The determination of mercury in whole blood and urine by inductively coupled plasma mass spectrometry
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DOI：
10.1016/s0584-8547(99)00057-9
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发表时间：
1999-08-09
影响因子：
3.3
通讯作者：
Moyer, TP
中科院分区：
文献类型：
--
作者：
Nixon, DE;Burritt, MF;Moyer, TP
建立了电感耦合质谱法测定全血和尿中汞的方法。金和重铬酸在盐酸中作为减少汞喷雾室记忆的试剂进行了评估。结果表明，重铬酸盐法优于金法。我们评估了快速引入样品以促进平衡，以及在样品分析后快速引入洗涤液以尽量减少汞记忆。在每个样品洗涤周期的开始和结束时，对20名S使用这种“快速泵”模式(2.5毫升/分钟)。稀释前原样品中汞的检出限为0.15微克/L。魁北克毒理中心实验室间比对样品的电感耦合等离子体质谱与目标浓度的回归和相关系数为：全血：y=1.0x-0.6；r=0.9801；n=27；尿液：y=0.84x+8；r=0.9915；n=42。采用电感耦合等离子体质谱(ICPMS)和冷原子吸收光谱仪(CVAAS)对样品进行分析。患者样本的回归和相关系数分别为：尿液Y=0.93X+1；r=0.8763；全血：Y=1.1X+0.2；r=0.9357；电感耦合等离子体质谱(ICMS)与电感耦合等离子体原子吸收光谱分析(CVAAS)测定29份尿样汞含量的相关性为：y=0.94x+4，r=0.9864。(C)1999 Elsevier Science B.V.保留所有权利。
An inductively coupled mass spectrometric (ICP-MS) method for the determination of mercury in whole blood and urine was developed. Gold and dichromate in hydrochloric acid were evaluated as agents to reduce mercury spray chamber memory. Dichromate with hydrochloric acid was found to be superior to gold. We evaluated the rapid introduction of sample to promote equilibrium and the rapid introduction of wash solutions after the sample analyses to minimize mercury memory. This 'fast pump' mode (2.5 ml/min) was used for 20 s at the beginning and end of each sample-wash cycle. The mercury detection limit is 0.15 mu g/l in the original sample before dilution. Regressions and correlation coefficients for ICP-MS vs. target concentrations for interlaboratory comparison samples from the Centre de Toxicologie du Quebec were: whole blood: y = 1.0x - 0.6; r = 0.9801; n = 27 and urine: y = 0.84x + 8; r = 0.9915; n = 42. Patient samples were analyzed by ICP-MS and cold vapor atomic absorption spectrometry (CVAAS). Regressions and correlations for patient samples were: urines: y = 0.93x + 1; r = 0.8763; n = 456 and whole blood: y = 1.1x + 0.2; r = 0.9357; n = 251. ICP-MS correlation with CVAAS for 29 urine samples containing 15-150 mu g Hg/specimen was: y = 0.94x + 4; r = 0.9864. (C) 1999 Elsevier Science B.V. All rights reserved.