Plasma total antioxidant capacity in hemodialyzed patients and its relationships to other biomarkers of oxidative stress and lipid peroxidation
Plasma total antioxidant capacity in hemodialyzed patients and its relationships to other biomarkers of oxidative stress and lipid peroxidation
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DOI：
10.1515/cclm.2002.019
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发表时间：
2002-01-01
影响因子：
6.8
通讯作者：
Di Lorenzo, D
中科院分区：
文献类型：
--
作者：
Gerardi, G;Usberti, M;Di Lorenzo, D
接受长期血液透析（HD）的患者表现出更高的氧化应激水平，可能导致心血管疾病的发病率增加。本研究代表了一些最广泛使用的氧化指标的关键评估，应用于血液透析相关氧化应激的监测。血浆总抗氧化能力通过两种独立的方法测定，即总抗氧化状态（TAS）和血浆铁还原能力（FRAP）。通过测定过氧化产物丙二醛和4-羟基壬烯醛（MDA-4HNE）以及脂质氢过氧化物（“Fox-2”和“d-ROMs”方法）来评估血浆脂质过氧化。同时测定血浆总硫醇和血浆a-生育酚。HD患者的MDA-4HNE水平较高，HD患者的MDA-4HNE水平降低，可能是由于透析滤过器的被动扩散。HD患者的d- rom也较高，但在透析后进一步增加。血清α -生育酚无显著性差异。HD患者的血浆硫醇较低，并在HD后恢复。与对照组相比，两种方法测定的血浆总抗氧化能力在HD患者中出乎意料地更高，并且在HD后下降。这些数据表明，在所研究的生物标志物中，d- rom水平更准确地反映HD患者发生的氧化改变，而MDA-4HNE的测定无法检测HD期间发生的氧化。此外，我们的研究结果指出，HD患者总抗氧化能力的测定受到伴随的血浆尿酸水平波动的严重影响，因此需要仔细解释。
Patients undergoing long-term hemodialysis (HD) exhibit increased levels of oxidative stress, likely contributing to the increased rate of cardiovascular disease. The present study represents a critical evaluation of some of the most widely used oxidative indicators, as applied to the monitoring of hemodialysis-associated oxidative stress.Total plasma antioxidant capacity was determined by two independent procedures, the total antioxidant status (TAS) and the ferric reducing ability of plasma (FRAP) methods. Plasma lipid peroxidation was assessed by determining the peroxidation products malonaldehyde and 4-hydroxynonenal (MDA-4HNE) as well as lipid hydroperoxides ("Fox-2" and "d-ROMs" methods). Total plasma thiols and plasma a-tocopherol were also determined.MDA-4HNE levels were higher in HD patients and decreased following HD, possibly due to passive diffusion across dialysis filters. d-ROMs were also higher in HD patients but exhibited a further increase following the dialysis procedure. Serum alpha-tocopherol did not show any significant differences. Plasma thiols were lower in HD patients and were restored following HD. Plasma total antioxidant capacity determined with either method was unexpectedly higher in HD patients compared to controls, and decreased following HD.These data indicate that, of the biomarkers studied, d-ROMs level is the one more accurately reflecting the oxidative alterations taking place in HD patients, while determination of MDA-4HNE fails to detect oxidation occurring during the HD sessions. In addition, our findings point out that the determination of total antioxidant capacity in HD patients is severely affected by the concomitant fluctuations in plasma urate levels and therefore needs careful interpretation.